裂果薯总皂苷制备工艺优化及其质量控制方法研究
Optimization of Preparing Technology of Total Saponins form Schizocapsa plantaginea Hance and its Quality Control Method
李昊 1李锦华 1莫宇雪 1吕美娴 1郭心怡 1梁梓樱 1王燕雪 1刘布鸣 2梁钢 3袁建童2
作者信息
- 1. 广西医科大学药学院,南宁 530021
- 2. 广西中药质量标准研究重点实验室,南宁 530015
- 3. 广西医科大学药学院,南宁 530021;广西中药质量标准研究重点实验室,南宁 530015;广西医科大学,靶向肿瘤学国家重点实验室,南宁 530021
- 折叠
摘要
目的 建立从裂果薯药材中制备总皂苷的方法,并建立总皂苷的质量控制方法.方法 采用水浴回流的方法从裂果薯块茎粉末中提取总皂苷粗提取物,用溶剂萃取和大孔树脂对粗提物进行富集纯化,制成裂果薯总皂苷(SSPHs),分别建立薄层色谱法、紫外可见分光光度法和超高效液相色谱-串联质谱(UHPLC-MS)联用对SSPHs进行质量评价.结果 所建立的制备方法从裂果薯块茎中制备总皂苷制品得率0.29％～2.81％,总皂苷纯度57.30％～77.02％,经检测其中含有裂果薯皂苷Ⅰ(SSPH Ⅰ)和裂果薯皂苷Ⅱ(SSPH Ⅱ)等皂苷类物质,SSPH Ⅰ含量为 10.77～85.60 mg·g-1,SSPH Ⅱ含量为 0.19～4.75 mg.g-1.结论 所建立的制备方法简单、高效、环保,产品品质高,所建立的定性、定量检测方法能有效反映SSPHs的质量情况,可为裂果薯总皂苷的质量控制提供方法参考.
Abstract
OBJECTIVE To establish a method for preparing total saponins from Schizocapsa plantaginea Hance and establish a corresponding quality control method.METHODS The total saponins were extracted from the Schizocapsa plantaginea Hance tubers using a water bath reflux method,followed by purification of the crude extract through solvent extraction and macroporous resin.Subse-quently,the purified product of total saponins of Schizocapsa plantaginea Hance(SSPHs)was prepared,and its quality was assessed using thin-layer chromatography,UV-visible spectrophotometry,and ultra-high performance liquid chromatography-mass spectrometry(UHPLC-MS)combined method.RESULTS The developed preparation method resulted in a yield of total saponin products from the Schizocapsa plantaginea Hance tuber ranging from 0.29％to 2.81％,with a purity from 57.30％to 77.02％.After detection,saponin substances such as Schizocapsa Plantaginea Hance Ⅰ(SSPH Ⅰ)and Schizocapsa plantaginea Hance Ⅱ(SSPH Ⅱ)were found in the total saponin products.The content of SSPH Ⅰ was 10.77-85.60 mg·g-1 and the content of SSPH Ⅱ was 0.19-4.75 mg·g-1.CONCLUSION The established preparation method is simple,efficient,and environmentally friendly,with high product quality.The established qualitative and quantitative detection method can effectively reflect the quality of SSPHs and provide method reference for the quality control of SSPHs.
关键词
裂果薯/总皂苷/超高效液相-串联质谱/质量控制Key words
Schizocapsa plantaginea Hance/total saponin/UHPLC-MS/MS/quality control引用本文复制引用
出版年
2024
NETL
NSTL
万方数据