Optimization of Preparing Technology of Total Saponins form Schizocapsa plantaginea Hance and its Quality Control Method
OBJECTIVE To establish a method for preparing total saponins from Schizocapsa plantaginea Hance and establish a corresponding quality control method.METHODS The total saponins were extracted from the Schizocapsa plantaginea Hance tubers using a water bath reflux method,followed by purification of the crude extract through solvent extraction and macroporous resin.Subse-quently,the purified product of total saponins of Schizocapsa plantaginea Hance(SSPHs)was prepared,and its quality was assessed using thin-layer chromatography,UV-visible spectrophotometry,and ultra-high performance liquid chromatography-mass spectrometry(UHPLC-MS)combined method.RESULTS The developed preparation method resulted in a yield of total saponin products from the Schizocapsa plantaginea Hance tuber ranging from 0.29％to 2.81％,with a purity from 57.30％to 77.02％.After detection,saponin substances such as Schizocapsa Plantaginea Hance Ⅰ(SSPH Ⅰ)and Schizocapsa plantaginea Hance Ⅱ(SSPH Ⅱ)were found in the total saponin products.The content of SSPH Ⅰ was 10.77-85.60 mg·g-1 and the content of SSPH Ⅱ was 0.19-4.75 mg·g-1.CONCLUSION The established preparation method is simple,efficient,and environmentally friendly,with high product quality.The established qualitative and quantitative detection method can effectively reflect the quality of SSPHs and provide method reference for the quality control of SSPHs.
Schizocapsa plantaginea Hancetotal saponinUHPLC-MS/MSquality control
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