Chemical Constituents from Illigera celebica and Their Cytotoxicity Activity
OBJECTIVE To isolate and identify the chemical constituents of the ethnic medicinal plant Illigera celebica and their cytotoxic activities.METHODS The ethyl acetate extracts of I.celebica were isolate and purified by silica gel,Rp-18,Sephadex LH-20 gel column chromatographies and preparative HPLC method.The chemical structures of the isolates were illustrated by compari-son their physicochemical properties and spectroscopic data with those reported.The cytotoxicity was evaluated by MTT method.RESULTS Fourteen compounds were isolated and identified as actinodaphnine(1),norisoboldine(2),atheroline(3),pityriacitrin(4),3,3',4'-tri-O-methylellagic acid(5),(+)-syringaresinol(6),(+)-pinoresinol(7),cleomiscosin A(8),stigmasta-4,6,8(14),22-tetraen-3-one(9),liquiritigenin(10),scopoletin(11),isofraxidin(12),physcion(13)and chrysophamol(14).The tested compounds showed no significant cytotoxic activity against the MGC-803,T-47D and T-24 cell lines.CONCLUSION All the isolates are obtained from this medicinal plant for the first time,and compounds 11-13 are obtained from Illigera genus for the first time.