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宽药青藤化学成分及其细胞毒活性研究

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目的 研究民族药用植物宽药青藤的化学成分及其细胞毒活性.方法 运用正相、反相硅胶,Sephadex LH-20凝胶等分离材料以及高效液相色谱法对宽药青藤乙酸乙酯层提取物的化学成分进行分离,通过核磁共振、质谱等光谱数据和物理化学性质鉴定化合物结构,并对分离得到的化合物进行细胞毒活性测试.结果 从宽药青藤中共分离得到14个化合物,分别为 actinodaphnine(1)、norisoboldine(2)、芒籽香碱(3)、pityriacitrin(4)、3,3',4'-三甲氧基鞣花酸(5)、(+)-丁香脂素(6)、(+)-松脂素(7)、臭矢菜素A(8)、豆甾-4,6,8(14),22-四烯-3-酮(9)、甘草素(10)、莨菪亭(11)、异嗪皮啶(12)、大黄素甲醚(13)和大黄酚(14);四唑盐(MTT)比色法结果显示,所测试的10个化合物对MGC-803、T-47D及T-24细胞增殖无明显抑制作用.结论 所有化合物均为首次从该植物中分离得到,化合物11~13为首次从该属植物中得到.
Chemical Constituents from Illigera celebica and Their Cytotoxicity Activity
OBJECTIVE To isolate and identify the chemical constituents of the ethnic medicinal plant Illigera celebica and their cytotoxic activities.METHODS The ethyl acetate extracts of I.celebica were isolate and purified by silica gel,Rp-18,Sephadex LH-20 gel column chromatographies and preparative HPLC method.The chemical structures of the isolates were illustrated by compari-son their physicochemical properties and spectroscopic data with those reported.The cytotoxicity was evaluated by MTT method.RESULTS Fourteen compounds were isolated and identified as actinodaphnine(1),norisoboldine(2),atheroline(3),pityriacitrin(4),3,3',4'-tri-O-methylellagic acid(5),(+)-syringaresinol(6),(+)-pinoresinol(7),cleomiscosin A(8),stigmasta-4,6,8(14),22-tetraen-3-one(9),liquiritigenin(10),scopoletin(11),isofraxidin(12),physcion(13)and chrysophamol(14).The tested compounds showed no significant cytotoxic activity against the MGC-803,T-47D and T-24 cell lines.CONCLUSION All the isolates are obtained from this medicinal plant for the first time,and compounds 11-13 are obtained from Illigera genus for the first time.

HernandiaceaeIlligera celebicaatherolinecytotoxic activity

徐娟娟、黄小芳、谭钦刚

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桂林医学院药学院,广西桂林 541199

中国科学院昆明植物研究所,植物化学与西部植物资源持续利用国家重点实验室,昆明 650201

莲叶桐科 宽药青藤 芒籽香碱 细胞毒活性

2024

中国药学杂志
中国药学会

中国药学杂志

CSTPCD北大核心
影响因子:0.957
ISSN:1001-2494
年,卷(期):2024.59(24)