Effect of Salvia Miltiorrhiza Polysaccharide on the Proliferation,Migration and Apoptosis of Lung Cancer Cells A549 Through MAPK/ERK Signal Axis Inhibition
Objective To investigate the effect of Salvia miltiorrhiza polysaccharide on proliferation,migration and apoptosis of lung cancer cells A549 through mitogen active protein kinase(MAPK)/extracellular signal-regulated kinase(ERK)signal axis inhibition.Methods A549 cells were cultured in vitro and treated with different mass concentrations(0,1,2,4,8,16,32,64 mg/mL)of Salvia miltiorrhiza polysaccharides for 48 h to determine the half inhibitory concentration(IC50)of Salvia miltiorrhiza polysaccharides.A549 cells were divided into the control group,high-dose group,medium-dose group,and low-dose group(0,8,4,2 mg/mL of Salvia miltiorrhiza polysaccharide),and the inhibitor group(40 µmol/L PD 98059).The migration of A549 cells was detected by the scratch healing assay,and the apoptosis of A549 cells was detected by the flow cytometry.The mRNA and protein expression levels of matrix metalloproteinase-9(MMP-9),MAPK,ERK,B cell lymphoma-2(Bcl-2)and caspase-3 were detected by the reverse transcription-polymerase chain reaction(RT-PCR)and the Western blot respectively.Results With the increase of the mass concentration of Salvia miltiorrhiza polysaccharides,the proliferation rate of A549 cells significantly decreased(P＜0.05).The IC50 of Salvia miltiorrhiza polysaccharides on A549 cells was 7.82 mg/mL,and 8,4,and 2 mg/mL were selected as the intervention doses for high-,medium-,and low-dose groups respectively.Compared with those in the control group,the migration distance of cells,MMP-9,MAPK,ERK,and Bcl-2 mRNA and protein expression levels significantly decreased(P＜0.05),while the apoptosis rate and caspase-3 mRNA and protein expression levels significantly increased in the inhibitor group and the high-,medium-,and low-dose groups(P＜0.05).Compared with those in the inhibitor group,the migration distance of cells,MMP-9,MAPK,ERK,and Bcl-2 mRNA and protein expression levels significantly increased(P＜0.05),while the apoptosis rate and caspase-3 mRNA and protein expression levels significantly decreased in the high-,medium-,and low-dose groups(P＜0.05),which showed a dose-dependent relationship with the increase of Salvia miltiorrhiza polysaccharide dose(P＜0.05).Conclusion Salvia miltiorrhiza polysaccharide can induce apoptosis of A549 cells and inhibit their proliferation and migration through the MAPK/ERK signaling axis.
Salvia miltiorrhiza polysaccharideMAPK/ERK signal axislung cancercell proliferationcell migrationcell apoptosis
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