Comparison of Rhei Radix et Rhizoma-Salviae Miltiorrhizae Radix et Rhizoma with Different Compatibility Proportions on Dissolution Rate of Active Ingredients and Anti-Renal Fibrosis
Objective To compare Rhei Radix et Rhizoma-Salviae Miltiorrhizae Radix et Rhizoma drug pair with different compatibility proportions on the dissolution rate of seven active ingredients(aloe-emodin,rhein,emodin,chrysophanol,physcion,salvianolic acid B and tanshinone ⅡA)and anti-renal fibrosis in rats.Methods High-performance liquid chromatography(HPLC)method was used to determine the content and dissolution rate of seven active ingredients in single extract of Rhei Radix et Rhizoma,Salviae Miltiorrhizae Radix et Rhizoma and mixed extract of drug pair with six compatibility proportions(1∶1,1∶1.25,1∶1.75,1∶2,1∶3 and 1∶4).Rat models of renal fibrosis were replicated by unilateral ureteral obstruction.The effects of single extract of Rhei Radix et Rhizoma,Salviae Miltiorrhizae Radix et Rhizoma and mixed extract of drug pair with three compatibility proportions(1∶1.25,1∶1.75 and 1∶2)on the pathological morphology of renal tissue,blood urea nitrogen(BUN),serum creatinine(SCr)and 24-hour urine total protein(24 h-UTP)levels in rats were compared.Results The content of seven active ingredients in the mixed extract of Rhei Radix et Rhizoma-Salviae Miltiorrhizae Radix et Rhizoma with different compatibility proportions was lower than that in the above two single extract.The overall dissolution rate of active ingredients in the mixed extract of drug pair at a compatibility proportion of 1∶2 was better than that at other compatibility proportions.Compared with that in the model group,the degree of renal fibrosis in rats significantly decreased under the single extract of Rhei Radix et Rhizoma,Salviae Miltiorrhizae Radix et Rhizoma and mixed extract of drug pair with three compatibility proportions(1∶1.25,1∶1.75 and 1∶2),and the BUN,SCr and 24 h-UTP levels significantly decreased,with the most obvious improvement under the extract of Rhei Radix et Rhizoma.Conclusion The established method can be used for the content determination of seven active ingredients in Rhei Radix et Rhizoma-Salviae Miltiorrhizae Radix et Rhizoma drug pair.The dissolution rate of active ingredients in the drug pair with different compatibility proportions is lower than that in the two single extract.The compatibility of Rhei Radix et Rhizoma and Salviae Miltiorrhizae Radix et Rhizoma do not have a synergistic effect on the anti-renal fibrosis,and the extract of Rhei Radix et Rhizoma is the most effective.
Rhei Radix et RhizomaSalviae Miltiorrhizae Radix et Rhizomacompatibility proportiondissolution raterenal fibrosisrat
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