Simultaneous Detection of 12 Flavonoid Glycosides in Aurantii Fructus Immaturus and Aurantii Fructus by HPLC
Objective To establish a high-performance liquid chromatography method for simultaneous detection of 12 flavonoid glycosides in Aurantii Fructus Immaturus and Aurantii Fructus.Methods The chromatographic column was Agilent Zorbax SB-C18 column(250 mm×4.6 mm,5 μm),the mobile phase was 0.1%formic acid aqueous solution-acetonitrile(gradient elution),the flow rate was 1.0 mL/min,the detection wavelength was 284 nm(eriocitrin,neoeriocitrin,narirutin,naringin,hesperidin,neohesperidin,poncirin,naringenin,hesperetin),330 nm(meranzin,nobiletin,tangeretin),the column temperature was 35℃,and the injection volume was 10 μL.Results The linear ranges of the above components were 0.19-46.61 μg/mL,0.17-43.27 μg/mL,0.20-51.07 μg/mL,11.34-756.01 μg/mL,0.41-101.28 μg/mL,20.58-1 028.79 μg/mL,0.20-50.49 μg/mL,0.10-25.25 μg/mL,0.10-26.63 μg/mL,0.24-11.96 μg/mL,0.21-52.64 μg/mL,0.55-27.51 μg/mL,respectively.The limit of detection(LOD)was not greater than 0.10 μg/mL,and the limit of quantification(LOQ)was not greater than 0.41 μg/mL.The RSDs of precision,stability and repeatability tests were all lower than 2.0%.The average recovery rates of above components were in the range of 92.90%to 114.33%,with the RSDs of 0.30%to 1.98%(n = 9).Conclusion The established method is simple and reliable,which can be used for the simultaneous detection of 12 flavonoid glycosides in Aurantii Fructus Immaturus and Aurantii Fructus.
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