Effect of 60Co-γ-Ray Irradiation on the Content of Luteoloside in Liyan Gargle
Objective To investigate the effect of 60Co-γ-ray irradiation on the content of luteoloside in Liyan Gargle.Methods High-performance liquid chromatography(HPLC)method was adopted.The chromatographic column was Agilent Zorbax Eclipse XDB-C18 column(250 mm×4.6 mm,5 μm),the mobile phase was acetonitrile-0.1%phosphoric acid aqueous solution(20∶80,V/V),the flow rate was 1.0 mL/min,the detection wavelength was 350 nm,the column temperature was 30℃,and the injection volume was 10 μL.The samples were irradiated at 6,10 kGy of 60Co-γ-ray respectively,and the content of luteoloside was determined.Results The linear range of luteoloside was 1.895 4-47.385 8 μg/mL(r = 0.999 7,n = 6).The limit of detection of luteoloside was 0.2 ng,and its limit of quantification was 0.4 ng.The RSDs of precision,stability and repeatability tests were all lower than 2.0%.The average recovery rate of luteoloside was 100.47%,with an RSD of 2.60%(n = 9).The average content of luteoloside in the samples was 23.890 1 μg/mL(0 kGy,before irradiation),16.269 3 μg/mL(after irradiation at 6 kGy)and 12.743 4 μg/mL(after irradiation at 10 kGy),with the RSDs of 2.73%,0.50%and 1.36%(n = 3)respectively.Based on the inter-batch and medicinal differences,the content limit of luteoloside in the preparation was tentatively 20 μg/mL.Conclusion The established method is easy,repeatable,specific and reliable,which can be used for quality evaluation of Liyan Gargle.60Co-γ-ray irradiation sterilization is not suitable for Liyan Gargle.
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