Chemical Constituents of Ethyl Acetate Layer Extracted from Hedyotis Scandens and Its Protective Effect on RGC-5 Cell Injury
Objective To analyze the chemical constituents of the ethyl acetate layer extracted from Hedyotis scandens,and to investigate its protective effect on RGC-5 cell injury.Methods The ethyl acetate layer extracted from Hedyotis scandens was isolated by the silica gel column,and MCI column,and it was purified by the preparative high-performance liquid chromatography(HPLC).The structure of the compound was identified by Magnetic Resonance Imaging(MRI)method,and the protective effect of the compound on propofol-induced RGC-5 cell injury was detected by the CCK-8 method.Results Fifteen compounds were isolated and identified,including capsaicin(compound 1),homodihydrocapsaicin Ⅰ(compound 2),secoisolariciresinol(compound 3),lariciresinol(compound 4),dehydrodiconiferyl alcohol(compound 5),cleomiscosin A(compound 6),curcasinlignan B(compound 7),syringaresinol(compound 8),oleanolic acid(compound 9),ursolic acid(compound 10),methyl 4-benzoyloxy-3-methoxybenzeneacetate(compound 11),(R)-6-methyl-4,6-bis(4-methylpent-3-enyl)cyclohexa-1,3-dienecarbaldehyde(compound 12),2-hydroxy-1-methoxy-anthraquinone(compound 13),isoscopoletin(compound 14),1H-indole-3-carboxaldehyde(compound 15).Compounds 1,4,6-8 had certain protective effects on the propofol-induced RGC-5 cell injury,among which compounds 4 and 7 had the most significant activity,and the cell viability was increased by 15.89%and 13.09%,respectively.Conclusion Compounds 1-7 were isolated and discovered for the first time from the genus Hedyotis,while compounds 9-10 and 13 were isolated and discovered for the first time from Hedyotis scandens.Compounds 4 and 7 showed good protective effects on the propofol-induced RGC-5 cell injury.
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