Objective To establish the quality standard for Chinese Herbal Gargle Granules.Methods Thin-layer chromatography(TLC)was used to qualitatively identify the ingredients in the prescription,including Drynariae Rhizoma,Taraxaci Herba,Viole Herba,and Glycyrrhizae Radix et Rhizoma.High-performance liquid chromatography(HPLC)was used to determine the content of quercetin in the preparation,the chromatographic column was Welch Ultimate XB C18 column(250 mm×4.6 mm,5 µm),the mobile phase was acetonitrile-0.1%formic acid solution(10∶90,V/V),the flow rate was 1.0 mL/min,the detection wavelength was 344 nm,the column temperature was 30℃,and the injection volume was 10 µL.Results The TLC identification spots of Drynariae Rhizoma,Taraxaci Herba,Viole Herba,and Glycyrrhizae Radix et Rhizoma were clear,with good separation,and the negative control had no interference.The linear range of quercetin was 9.2-64.4 µg/mL(R2 = 0.999 7,n = 7).The RSDs of precision,stability,and repeatability test results were all lower than 4.0%.The average recovery rate of quercetin was 104.65%with an RSD of 4.60%(n = 6).The content of quercetin in three batches of samples were 0.907,0.851,0.817 mg/g(n = 3),respectively.Conclusion The established method is simple and reproducible,which can be used for quality control of Chinese Herbal Gargle Granules.
关键词
中草药漱口颗粒/秦皮乙素/薄层色谱法/高效液相色谱法/质量标准
Key words
Chinese Herbal Gargle Granules/quercetin/TLC/HPLC/quality standard
维普
万方数据