Effects of Rumicis Obtusifolii Radix on Anxiety-Like Behavior of Model Mice with Psoriasis
Objective To investigate the effect of Rumicis Obtusifolii Radix on the anxiety-like behavior of model mice with psoriasis.Methods Model mice with anxiety were replicated by multiple stimulation,and imiquimod cream preparation was applied to the depilated area on the back of mice to replicate the model mice with anxiety and psoriasis.A total of 90 model mice were divided into the model group(equal volume of physiological saline),the methotrexate group(1.3 mg/kg),the aprepitant group(5 mg/kg),the Rumicis Obtusifolii Radix low-,medium-and high-dose groups(1,2,4 g/kg),with 15 mice in each group.The mice in each group were given corresponding drugs or physiological saline by gavage once a day for 15 d.Another 15 mice without stimulation were selected as the normal control group,vaseline was applied to the depilated area on the back,and an equal volume of physiological saline was given by gavage.The brain,heart,liver,adrenal gland,spleen and thymus of mice were weighed,and the organ coefficients were calculated.The behavioral test was conducted.The skin pathological morphology on the back of mice was observed and scored.Results Compared with those in the model group,the liver coefficient in the aprepitant group and Rumicis Obtusifolii Radix high-dose group and the kidney coefficient in the Rumicis Obtusifolii Radix medium-dose group significantly decreased(P<0.05);the movement distance in the central area,average rate in the central and edge area,frequency of entry into the central area in the each dose group of Rumicis Obtusifolii Radix significantly increased,and the incubation period significantly prolonged(P<0.05).Compared with those in the model group,the percentage of residence time at the novel arm and start arm in the methotrexate group,aprepitant group,the Rumicis Obtusifolii Radix medium-and high-dose groups significantly increased,while the percentage of residence time at other arm significantly decreased(P<0.05);the stratum dermis score and Baker score in the each dose group of Rumicis Obtusifolii Radix,the stratum epidermis score in the Rumicis Obtusifolii Radix medium-and high-dose groups,and the stratum corneum score in the Rumicis Obtusifolii Radix low-and high-dose groups significantly decreased(P<0.05).Compared with those in the model group,the stratum spinosum thickening,parakeratosis and hyperkeratosis of the back skin in the methotrexate group,the aprepitant group and the each dose group of Rumicis Obtusifolii Radix improved,there was no obvious extension of epidermal protrusion and upward movement of dermal papillae,and the infiltration of subcutaneous inflammatory cells improved.Conclusion Rumicis Obtusifolii Radix can relieve the injuries of stratum corneum,stratum epidermis,stratum dermis of mice with psoriasis,and improve their anxiety and cognitive impairment.
万方数据
维普
