Mechanism Study of Gualoupi Injection in the Treatment of Stable Angina Pectoris Based on Network Pharmacology
Objective To investigate the mechanism of Gualoupi Injection in the treatment of stable angina pectoris(SAP).Methods The relevant studies were searched to obtain the active ingredients of the preparation,and the SwissTargetPrediction database was used to predict their targets.The GeneCards database was used to obtain the SAP targets.The Venny 2.1 platform was used to obtain the common targets.The MetaScape software was used to perform the Gene Ontology(GO)functional enrichment analysis and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analysis on targets,the Cytoscape 3.9.1 software was used to construct an ingredient-target-pathway network,and the String database was used to construct a protein-protein interaction(PPI)network.The differentially expressed genes in the Gene Expression Omnibus(GEO)database and molecular docking was used for verification,and the PyMOL 2.3.0 software was used for visualization analysis.Results A total of 57 active ingredients were obtained from the preparation.There were 517 targets,of which 26 were related to SAP.The core active ingredients were flavonoids such as luteolin,rutin,cynaroside,apigenin-7-O-β-D-glucoside and diosmetin-7-O-β-glucoside,the core targets were TNF,FGF2,MMP-9,ICAM1,EGFR and others.The genes were mainly enriched in thirty-seven GO functions(including twenty biological processes,nine molecular functions,eight cellular components)and nine KEGG pathways.The verification showed that the active ingredients could specifically act on some differentially expressed genes of diseases.The binding energy of core active ingredients with targets was lower than-10.6 kcal/mol.Conclusion Gualoupi Injection may exert anti-inflammatory,antioxidant stress and anti-apoptotic effects through multiple active ingredients,specific targets and pathways,thereby exerting therapeutic effects on SAP.
万方数据
维普
