Prediction of Efficacy-Related Substances in Senecionis Scandentis Hebra
Objective To predict and analyze the efficacy-related substances in Senecionis Scandentis Hebra.Methods The high-performance liquid chromatography(HPLC)method was adopted.The chromatographic column was Shim-pack GIST C18 column(250 mm×4.6 mm,5 µm),the mobile phase was acetonitrile-0.1%phosphoric acid aqueous solution(gradient elution),the flow rate was 1.0 mL/min,the detection wavelength was 345 nm,the column temperature was 25℃,and the injection volume was 10 µL.The HPLC fingerprints of 15 batches of medicinal material samples were established,and the Similarity Evaluation System for Chromatographic Fingerprints of Traditional Chinese Medicine(Version 2012)was used for the similarity evaluation,and the common peaks were identified.Cluster analysis and principal component analysis were performed for the preliminary prediction of potential efficacy components.The potential targets of components were obtained,the protein-protein interaction(PPI)network was constructed,the Gene Ontology(GO)functional enrichment analysis and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analysis were performed,the components-targets-pathways network was constructed based on the network pharmacology technology to further predict the mechanism of potential efficacy-related components,core targets and pathways of Senecionis Scandentis Hebra.Results Eighteen common peaks were obtained,of which seven were identified as chlorogenic acid,caffeic acid,hyperoside,isoquercetin,rutin,isochlorogenic acid A and isochlorogenic acid C.The similarity of 15 batches of medicinal material samples was in the range of 0.892 to 0.997,with only one batch lower than 0.9.Fifteen batches of medicinal material samples could be grouped into two categories,and four main components were identified.A total of 109 targets and 18 key targets(including EGFR,JUN,ESR1,MMP-9 and so on)were associated with seven components(common peaks).A total of 233 GO entries were obtained,including 197 biological processes,17 cellular components,and 19 molecular functions,mainly involving cellular responses to radiation,membrane rafts and regulation of signal receptor activity.A total of 59 KEGG pathways were obtained,mainly involving tumor signaling pathway,endocrine resistance pathway,relaxin signaling pathway and others.Conclusion Senecionis Scandentis Hebra may exert its efficacy through multiple efficacy-related components such as caffeic acid,isoquercetin,hyperoside and their targets such as EGFR,ESR1,JUN and MMP-9.
万方数据
维普
