Improvement of Quality Standard of Guqitai Liniment
Objective To improve the quality standard of Guqitai Liniment.Methods Thin-layer chromatography(TLC)method was used to qualitatively identify Cynanchi Paniculati Radix et Rhizoma,Asari Radix et Rhizoma and Clematidis Radix et Rhizoma in the preparation.The high-performance liquid chromatography(HPLC)method was used to determine the contents of notoginsenoside R1,ginsenoside Rg1 and ginsenoside Re simultaneously;the chromatographic column was Shimadzu WondaSil Superb C18 column(250 mm×4.6 mm,5 µm),the mobile phase was acetonitrile-water(gradient elution),the flow rate was 1.0 mL/min,the detection wavelength was 203 nm,the column temperature was 25℃,and the injection volume was 20 µL.Results The TLC chromatograms of Cynanchi Paniculati Radix et Rhizoma,Asari Radix et Rhizoma and Clematidis Radix et Rhizoma had clear spots,and there was no interference from the negative reference.The linear ranges of notoginsenoside R1,ginsenoside Rg1 and ginsenoside Re were 0.281 0-5.465 2 µg(R2 = 1.000 0),0.479 5-9.207 7 µg(R2 = 0.999 8)and 0.073 2-1.485 8 µg(R2= 0.999 9)respectively.The RSDs of precision,stability and repeatability tests were all lower than 2.0%.The average recovery rates of the above three ingredients were 98.44%,97.34%,and 103.51%,with the RSDs of 0.81%,1.69%and 0.58%(n = 6)respectively.The average contents of notoginsenoside R1,ginsenoside Rg1 and ginsenoside Re in four batches of samples were 0.247 6,1.038 8,0.110 2 mg/mL,respectively.Conclusion The established method is specific and repeatable,which can be used for the quality control of Guqitai Liniment.
Guqitai Linimentquality standardHPLCTLCnotoginsenoside R1ginsenoside Rg1ginsenoside ReAsari Radix et Rhizoma
万方数据
维普
