Objective To establish a method for the detection of N-{1-[3-(Trifluoromethyl)phenyl]-2-propanyl}-1-butanamine in the products for weight loss(including Chinese patent medicines,health food and food).Methods Ultra-high performance liquid chromatography tandem triple-quadrupole linear ion-trap mass spectrometry(UPLC-QTRAP-MS/MS)method was adopted.The chromatographic column was Waters ACQUITY UPLC®HSS T3 column(100 mm×2.1 mm,1.8 µm),the mobile phase was 0.1%formic acid aqueous solution-0.1%formic acid-methanol(gradient elution),the flow rate was 0.3 mL/min,the detection wavelength was 215 nm,the column temperature was 30℃,and the injection volume was 1 µL.The electro-spray ionization with positive mode(ESI+)was adopted.Qualitative analysis was conducted based on the retention time and ion pairs.The standard curve was fitted by the multiple reaction monitoring(MRM)mode,and the content of detected N-{1-[3-(Trifluoromethyl)phenyl]-2-propanyl}-1-butanamine was calculated.Results The linear range of N-{1-[3-(Trifluoromethyl)phenyl]-2-propanyl}-1-butanamine was 0.1-50 ng/mL(r = 1.000 0).The limit of detection was 0.4 ng/g,and the limit of quantification was 1.2 ng/g.The RSDs of precision,stability and repeatability tests were all lower than 3.0%.The average recovery rate of N-{1-[3-(Trifluoromethyl)phenyl]-2-propanyl}-1-butanamine was 109.67%,with an RSD of 5.46%(n = 9).Conclusion The method can detect N-{1-[3-(Trifluoromethyl)phenyl]-2-propanyl}-1-butanamine specifically and rapidly in the products for weight loss,which can be used to the screening and confirmation of a large quantity of samples.
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