Optimization of Processed Aconitum Leucostomum Based on Rat Models of Rapid Arrhythmia
Objective To optimize the processed Aconitum leucostomum that can prevent rapid arrhythmia in model rats.Methods The Kazak processing method was adopted.The raw Aconitum leucostomum was soaked for 12 h and 24 h respectively,and then boiled together with Glycyrrhizae Radix et Rhizoma and Sojae Semen Nigrum for 1,2,3,4 h to obtain eight processed products.A total of 96 rats were randomly divided into the blank control group(equal volume of physiological saline),the model group(equal volume of physiological saline),the raw Aconitum leucostomum group(referred to as the raw drug group,0.4 g/kg),the eight processed Aconitum leucostomum groups(1.2 g/kg,recorded as 12 h-1 h group,12 h-2 h group,12 h-3 h group,12 h-4 h group,24 h-1 h group,24 h-2 h group,24 h-3 h group,24 h-4 h group respectively),the amiodarone group(5 mg/kg),with eight rats in each group.The rats in each group were given corresponding drugs or physiological saline by gavage once a day for 14 d;after that,the blank control group was injected with an equal volume of physiological saline via the tail vein,while the other groups were injected with 10 mg/mL aconitine(3.125 mg/kg)to replicate the rapid arrhythmia models.The electrocardiogram of rats was detected,the pathological morphology of cardiac tissue in rats was observed by microscope,the superoxide dismutase(SOD),malondialdehyde(MDA),creatine kinase isoenzyme MB(CK-MB),cardiac troponin I(cTnI)levels were detected by the enzyme-linked immunosorbent assay,the activity of myocardial adenosine triphosphatase(ATPase)was detected,and the expression level of Cx45 protein in cardiac tissue was detected by the immunohistochemistry staining method.Results No ventricular fibrillation,ventricular tachycardia or premature beat was observed in the 24 h-1 h group,the premature beats and ventricular tachycardia in the other seven processed product groups and the amiodarone group occurred later than those in the model group.The myocardial fibers had disordered arrangement and obvious rupture in the model group;while those were better in the eight processed product groups and amiodarone group,and the edema and damage of myocardial cells relieved.Compared with those in the model group,the expression levels of SOD and Cx45 proteins of myocardial tissues in the eight processed product groups and the Ca2 +-ATPase level in the 24 h-2 h group and 24 h-3 h group were significantly higher(P＜0.05);the serum MDA level in the raw drug group,12 h-1 h group,12 h-2 h group and 24 h-1 h group was significantly lower(P＜0.05);the serum cTnI level in all the drug groups(except for the 24 h-2 h group and 24 h-3 h group)was significantly lower(P＜0.05);and the serum CK-MB level in all the drug groups(except for the 24 h-3 h group)was significantly lower(P＜0.05).Conclusion The processed Aconitum leucostomum by Kazak processing method have a certain preventive effect on the rapid arrhythmia in model rats,among which the 24 h-1 h processed product is more effective.
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