Simultaneous Determination of Six Components in Oral Five Vitamins,Lysine and Glucose by HPLC
Objective To establish a high-performance liquid chromatography(HPLC)method for simultaneous determination of six components in Oral Five Vitamins,Lysine and Glucose.Methods The chromatographic column was Alltima C18 column(250 mm×4.6 mm,5 μm),the mobile phase was 5 mmol/L sodium 1-heptanesulfonate solution(adjusted to pH 2.5 with 20%phosphoric acid aqueous solution)-acetonitrile(gradient elution),the flow rate was 1.0 mL/min,the detection wavelengths were 200 nm(calcium pantothenate,lysine hydrochloride),261 nm(niacinamide,vitamin B2,vitamin B1)and 290 nm(vitamin B6),the column temperature was 40℃,and the injection volume was 20 μL.Results The linear ranges of calcium pantothenate,niacinamide,vitamin B6,lysine hydrochloride,vitamin B2 and vitamin B1 were 1.174-23.49 μg/mL,4.220-84.40 μg/mL,0.520 7-10.41 μg/mL,20.88-417.7 μg/mL,0.427 8-8.555 μg/mL,1.676-33.52 μg/mL(r = 0.999 9)respectively.The RSDs of precision,stability and repeatability tests were all lower than 2.0%.The average recovery rates of the above six components were 101.02%,99.78%,100.30%,100.32%,95.61%,99.26%,with the RSDs of 0.99%,0.60%,0.57%,1.85%,1.69%,0.71%(n = 6)respectively.Conclusion This method is simple,accurate and reliable,which can be used to simultaneously determine the contents of six components in Oral Five Vitamins,Lysine and Glucose.
HPLCOral Five Vitamins,Lysine and Glucosecalcium pantothenateniacinamidevitamin B6lysine hydrochloridevitamin B2vitamin B1content determination
万方数据
维普
