Effect of Rivaroxaban on the Function of Human Umbilical Vein Endothelial Cells Induced by Oxidized Low-Density Lipoprotein
Objective To investigate the effect of rivaroxaban(RIV)on oxidative low-density lipoprotein(Ox-LDL)-induced functional impairment of human umbilical vein endothelial cells(HUVEC).Methods According to different treatments,cells were divided into the blank control group(group A,conventional culture medium),Ox-LDL group(group B,culture medium containing 100 μg/mL Ox-LDL),Ox-LDL + RIV125 group(group C1,culture medium containing 100 μg/mL Ox-LDL and 125 ng/mL RIV),Ox-LDL + RIV250 group(group C2,culture medium containing 100 μg/mL Ox-LDL and 250 ng/mL RIV)and Ox-LDL + RIV500 group(group C3,culture medium containing 100 μg/mL Ox-LDL and 500 ng/mL RIV).Cells in all groups were treated for 48 h.CCK-8 method was used to detect the changes in cell survival in different groups,flow cytometry was used to detect apoptosis,enzyme-linked immunosorbent assay(ELISA)was used to detect the levels of interleukin(IL)-1β,IL-6 and tumor necrosis factor-α(TNF-α),and Western blot was used to determine the expression levels of nuclear factor-κB(NF-κB)and urokinase-type plasminogen activator receptor(uPAR)proteins.Results Compared with those in group B,the cell survival rates in groups C1,C2,and C3 significantly increased(P<0.05),while the cell apoptosis rates and the expression level of uPAR in groups C1,C2,and C3 significantly reduced(P<0.05);the levels of IL-1β,TNF-α,IL-6,and the expression level of p-NF-κB p65 in the group C3 significantly reduced(P<0.05).Conclusion RIV can significantly restore cell viability,reduce cell apoptosis,and induce inflammatory reactions induced by Ox-LDL,which may play a role through the regulation of NF-κB and uPAR.
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