Effect of Matrine on Apoptosis of Osteosarcoma Cells
Objective To investigate the effect and mechanism of matrine in promoting apoptosis in MG-63 cells.Methods The experiment was divided into the non-matrine group and the 10%matrine group,and the rats in the two groups were orally administered normal saline and 10%matrine respectively to prepare drug-contained serum,and human osteosarcoma cell line MG-63 was intervened.CCK-8 method was used to detect cell proliferation.Flow cytometry was used to detect apoptosis.Fluorescence quantitative PCR was used to detect the expression of c-myc and caspase-9 genes.The expressions of related proteins Nur77,mitogen-activated protein kinase 5(MEK5),and apoptosis-related proteins c-myc and caspase-9 in extracellular signal-regulated kinase 5(ERK5)signaling pathway were detected by Western blot.Results Compared with the non-matrine group,the proliferation of MG-63 cells were significantly inhibited and their apoptosis were promoted in the 10%matrine group.The results of fluorescence quantitative PCR experiments showed that 10%matrine could inhibit c-myc transcription and promote caspase-9 transcription.Western blot experiments showed that in the ERK5 signaling pathway,10%matrine could downregulate the expression of Nur77,mitogen-activated protein kinase 5(MEK5),and c-myc protein,and upregulate the expression of caspase-9 protein.Conclusion Matrine may inhibit the proliferation of MG-63 cells and promote their apoptosis through interfering with the ERK5 signaling pathway and regulating the expression of proteins such as c-myc and caspase-9.
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