Objective To optimize the alcohol extraction and water precipitation process of Ginkgo biloba extract(GBE).Methods Based on the one-factor experiment,the transfer rate of total flavonol glycosides,and terpene lactones,and the dry matter yield were taken as comprehensive evaluation indicators,the liquid-solid ratio,extraction time,extraction times,concentration times of extraction liquid,water addition times and cryoprecipitation time were comprehensively investigated by the Box-Behnken Design(BBD)-response surface methodology(RSM).Results The optimal alcohol extraction and water precipitation process was as follows:the liquid-solid ratio was 8∶1(V/m),the extraction time was 1.0 h,the extraction times were three times,the concentration times were three times,the water addition times was three times,and the cryoprecipitation time was 28.0 h.Conclusion The optimal alcohol extraction and water precipitation process of GBE was designed by BBD-RSM,which is scientific,reasonable,stable and feasible.
Ginkgo biloba extract/alcohol extraction and water precipitation process/BBD-RSM/transfer rate of total flavonol glycoside/transfer rate of terpene lactonee/dry matter yield
维普
万方数据