首页|Box-Behnken Design响应面法优化银杏叶提取物醇提水沉工艺

Box-Behnken Design响应面法优化银杏叶提取物醇提水沉工艺

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目的 优化银杏叶提取物的醇提水沉工艺。方法 在单因素试验基础上，以总黄酮醇苷转移率、萜类内酯转移率及干物质得率为综合评价指标，通过Box-Behnken Design(BBD)响应面法对液料比、提取时间、提取次数、提取液浓缩倍数、加水倍数、冷沉时间进行考察。结果 醇提水沉最佳工艺为液料比 8:1(V/m)，提取时间 1。0h，提取次数 3 次，浓缩倍数 3 倍，加水倍数 3 倍，冷沉时间28。0 h。结论 BBD响应面法优化得到的银杏叶提取物醇提水沉工艺科学、合理、稳定、可行。
Optimization of Alcohol Extraction and Water Precipitation Process of Ginkgo Biloba Extract by the Box-Behnken Design-Response Surface Methodology
Objective To optimize the alcohol extraction and water precipitation process of Ginkgo biloba extract(GBE).Methods Based on the one-factor experiment,the transfer rate of total flavonol glycosides,and terpene lactones,and the dry matter yield were taken as comprehensive evaluation indicators,the liquid-solid ratio,extraction time,extraction times,concentration times of extraction liquid,water addition times and cryoprecipitation time were comprehensively investigated by the Box-Behnken Design(BBD)-response surface methodology(RSM).Results The optimal alcohol extraction and water precipitation process was as follows:the liquid-solid ratio was 8∶1(V/m),the extraction time was 1.0 h,the extraction times were three times,the concentration times were three times,the water addition times was three times,and the cryoprecipitation time was 28.0 h.Conclusion The optimal alcohol extraction and water precipitation process of GBE was designed by BBD-RSM,which is scientific,reasonable,stable and feasible.

Ginkgo biloba extractalcohol extraction and water precipitation processBBD-RSMtransfer rate of total flavonol glycosidetransfer rate of terpene lactoneedry matter yield

毕艳艳、姜佳峰、孙绍明、刘健、郭田甜、孙艳、张兰、郝大伟

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经方与现代中药融合创新全国重点实验室,山东 临沂 276006

鲁南厚普制药有限公司,山东 临沂 276006

鲁南制药集团股份有限公司,山东 临沂 276006

银杏叶提取物 醇提水沉工艺 Box-Behnken Design响应面法 总黄酮醇苷转移率 萜类内酯转移率 干物质得率

山东省重点研发计划(重大科技创新工程)山东省新旧动能转换重大产业攻关项目

2021CXGC010508鲁动能办[2021]23号

2024

10.3969/j.issn.1006-4931.2024.07.013

中国药业
重庆市食品药品监督管理局

中国药业

CSTPCD
影响因子：1.369
ISSN：1006-4931
年,卷(期)：2024.33(7)
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