Objective To improve the quality standard of Xingsu Zhike Granules.Methods Peucedani Radix,Pericarpium Citri Reticulatae and Platycodonis Radix in the preparation were identified qualitatively by the thin-layer chromatography(TLC).The content of praeruptorin A in the preparation was determined by the high-performance liquid chromatography(HPLC),the chromatographic column was Shiseido Capcell C18 column(250 mm×4.6 mm,5 μm),the mobile phase was methanol-water(65∶35,V/V),the flow rate was 1.0 mL/min,the detection wavelength was 321 nm,the column temperature was 35℃,and the injection volume was 10 μ L.Results The TLC spots of Peucedani Radix,Pericarpium Citri Reticulatae and Platycodonis Radix were clear and well separated,and the negative control had no interference.The linear range of praeruptorin A was 2.056-51.4 μg/mL(r = 0.999 9,n = 6).The RSDs of precision,stability and repeatability tests were lower than 3.0%(n = 6).The average recovery of praeruptorin A was 99.21%with an RSD of 2.01%(n = 6).The content of praeruptorin A in samples was in the range of 0.071 1-0.218 2 mg/g(n = 3).Conclusion The method is simple,reproducible and accurate,which can be used for quality control of Xingsu Zhike Granules.
Xingsu Zhike GranulesTLCHPLCpraeruptorin Aquality standard
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