Comparative Application of Three Mass Spectrometry Techniques in Multi-Component Analysis of Compound Fufangteng Mixture
Objective To compare the capabilities of quadrupole-exactive orbitrap mass spectrum(QE),quadrupole-time-of-flight high-resolution mass spectrum(Q-TOF)and triple quadrupole tandem mass spectrum(QQQ)for analyzing 13 compounds in Compound Fufangteng Mixture.Methods Mass spectrometry conditions:QE adopted the heated electric spray ionization(HESI)ion source and the Full MS/dd-MS2 mode for scanning with simultaneous positive and negative ions;Q-TOF adopted the electric spray ionization(ESI)ion source with positive and negative ions separately for full scanning;QQQ adopted ESI ion source and multi-reaction monitoring(MRM)mode with simultaneous positive and negative ions for full scanning.Chromatographic conditions:the chromatographic column was C18 column(100 mm×2.1 mm,1.9 μm),the mobile phase was acetonitrile(containing 0.1%formic acid)-0.1%formic acid(gradient elution),the flow rate was 0.3 mL/min,and the column temperature was 35℃.The applicability,qualitative screening ability,and quantitative result accuracy of three methods(three sample groups)were compared,and the principal component analysis(PCA)was conducted on the correlation and differences of various compounds among three groups by the SIMACA 14.1 multivariate statistical analysis software to draw PCA-X chart.Results The applicability of QE method was stronger than that of QQQ and Q-TOF,and the qualitative screening ability of QE and Q-TOF was stronger than that of QQQ.The PCA-X chart showed that the measurement results of QE and QQQ were both in the same position,and the distance was relatively close,showing a highly positive correlation.However,it was in the opposite position to the measurement results of Q-TOF,and the distance was far,showing a highly negative correlation.Conclusion Compared with Q-TOF and QQQ,QE is more suitable for the analysis of multi-components in Compound Fufangteng Mixture.
万方数据
维普
