Determination of Bisphenol A Residue in Intravenous Indwelling Needles by HPLC-MS/MS
Objective To establish a high-performance liquid chromatography-triple quadrupole tandem mass spectrometry(HPLC-MS/MS)method for the determination of bisphenol A residue in disposable intravenous indwelling needles.Methods Bisphenol A residue was extracted from the intravenous indwelling needle samples by ultrasound with acetonitrile as the solvent.The chromatography column was Waters Acquity Premier BEH C18 column(100 mm×2.1 mm,1.7 μm),the mobile phase was 0.05%ammonia water-methanol(28∶72,V/V),the flow rate was 0.3 mL/min,the column temperature was 30℃,and the injection volume was 2 μL.The triple quadrupole mass spectrometry adopted electric spray ionization ion source with negative ion and multi-reaction monitoring mode for scanning,the pressures of curtain gas,spray gas,auxiliary heating gas were 25,55,and 55 psi,respectively,the ionization voltage was 4 500 V,and the mass charge ratio(m/z)of quantitative ion was 227.1/133.0.Results The linear range of bisphenol A was 5.42-216.8 ng/mL(r = 0.999 9,n = 6).The limit of determination(LOD)was 0.108 4 ng/mL,and the limit of quantitation(LOQ)was 1.084 ng/mL.The RSDs of precision and repeatability test results were lower than 4.0%.The recovery rate of bisphenol A was 90.44%with an RSD of 1.27%(n = 6).The highest content of bisphenol A residue in commercial intravenous indwelling needle samples was 9 213.34 ng/g.Conclusion This method is simple,sensitive,reproducible,and accurate,which can be used for the determination of bisphenol A residue in disposable intravenous indwelling needles.
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