Content Determination of Total Polysaccharide in Brassica Rapa and the Fingerprint of Its Monosaccharides
Objective To establish an ultraviolet spectrometry method for the content determination of total polysaccharide in Brassica rapa,and the high-performance liquid chromatography(HPLC)fingerprint of itsmonosaccharides.Methods UV spectrophotometry was used to determine the content of total polysaccharide in six batches of samples at a wavelength of 484 nm,with D-anhydrous glucose as the reference substance and 5%phenol-sulfuric acid as the chromogenic agent.The fingerprint of monosaccharides was established by pre-column derivatization HPLC,the chromatographic column was Ultimate XB-C18 column(250 mm×4.6 mm,5 μm),the mobile phase was acetonitrile-0.1%phosphate solution with pH 6.8(gradient elution),the flow rate was 1.0 mL/min,the detection wavelength was 250 nm,the column temperature was 30℃,and the injection volume was 10 μL.The Similarity Evaluation System of Chromatographic Fingerprint of Traditional Chinese Medicine(Version 2012.0)was used to establish HPLC fingerprint of monosaccharides in six batches of Brassica rapa and conduct similarity evaluation.Common peaks were identified by comparing the monosaccharides with the reference substance.Results The linear range of D-anhydrous glucose was 1.84-18.37 mg/L(r = 0.999 5,n = 6),and the RSDs of precision,repeatability,and stability test results were all lower than 2.0%.The average recovery of D-anhydrous glucose was 100.88%with an RSD of 1.00(n = 6).The average content of total polysaccharides in six batches of samples was 285.48 mg/g.A total of 19 common peaks were identified in the HPLC fingerprint of monosaccharides,with peak 14 as galactose,peak 4 as D-mannose,peak 7 as rhamnose,peak 16 as arabinose,peak 13 as D-anhydrous glucose,peak 10 as D-glucuronic acid,and peak 12 as D-glucuronic acid,with similarity of 0.946-1.000.Conclusion The method is simple,stable and reliable,which can be used for the quality control and evaluation of total polysaccharides in Brassica rapa.
万方数据
维普
