Protective Effect of Harmine on Model Mice with Inflammatory Bowel Disease
Objective To investigate the protective effect of harmine on the intestinal barrier of model mice with inflammatory bowel disease(IBD).Methods A total of 64 C57BL/6J male mice were randomly divided into the blank control group(equal volume of water),the drug control group(0.05 mg/mL harmine),the model group(equal volume of water),and the drug observation group(0.05 mg/mL harmine),with 16 mice in each group.The mice were given the drinking water containing 2%dextran sulphate sodium for 7 d to replicate the IBD model,while the mice in the blank control group and drug control group were given the water.After successful modeling,the mice in each group were given corresponding drugs or water for 4 d.The survival rate,body mass and colon length of mice were observed.The pathological change of colon tissue was observed by the hematoxylin-eosin staining and scored.The expression levels of ZO-1 and Occludin proteins in the intestine of mice were detected by the Western blot.Results Compared with those in the model group,the survival rate significantly increased,the change rate of body weight significantly decreased,and the colon length significantly increased in the drug observation group(P<0.05);the epithelial injury and inflammatory cell infiltration of colon mucosa and submucosa significantly relieved,and pathological scores in the drug observation group significantly decreased(P<0.05);the expression levels of ZO-1 and Occludin proteins in the intestine of mice in the drug observation group significantly increased(P<0.05).Conclusion Harmine has a protective effect on the intestinal barrier of IBD model mice,and its mechanism may be related to increasing the expression levels of ZO-1 and Occludin proteins.
harmineinflammatory bowel diseaseintestinal barriermouseZO-1 proteinOccludin protein
国家科技期刊平台
NSTL
万方数据
维普
