Physical Characteristics and Bioactivity Analysis of Residues of Turkish Gall Treated by Ultra-Fine Grinding Combined with Semi-Bionic Technology
Objective To investigate the physical characteristics and changes in active ingredients of residues of Turkish gall(RTG)extracted by the semi-bionic technology and the antioxidant capacity of RTG.Methods RTG was processed through ultra-fine grinding and categorized into group A(<100 μm),group B(100-300 μm RTG),and group C(>300 μm RTG).according to the particle size.The physical characteristics of RTG were examined,including water,total ash content,water solubility,angle of repose,angle of slide,angle of collision,and bulk and vibrational densities,and other characteristics.RTG was treated with the semi-bionic technology with water and hydrochloric acid buffer to examine its effect on the proliferation and antioxidant capacity of human periodontal ligament fibroblast.Results With the increase of particle size,the moisture content significantly decreased(P<0.05),the total ash content significantly increased(P<0.05),the bulk density significantly increased(P<0.05),the angle of repose,angle of sliding and angle of collapse significantly decreased(P<0.05),while the vibrational density significantly increased(P>0.05).For the same particle size,the water-soluble index(WSI)of RTG increased with the increasing temperature(P<0.05).For the same temperature,the WSI of RTG decreased with increasing size(P<0.05).The total polyphenol content(TPC)in the hydrochloric acid buffer group was significantly higher than that in the water treatment group(P<0.05),and that in group A was significantly higher than that in group B and group C(P<0.05).The OD510 value in the hydrochloric acid buffer group was significantly higher than that in the water treatment group(P<0.05).The smaller the particle size,the more significant the proliferation effect of RTG on cells(P<0.05).The DPPH and hydroxyl radical scavenging activity in the hydrochloric acid buffer group were significantly better than those in the water treatment group(P<0.05).The smaller the particle size,the higher the antioxidant activity of RTG(P<0.05).Conclusion The reuse of RTG has great potential in the development of biotechnology products,which can provide a theoretical basis for improving the quality and clinical application of Turkish gall.
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