Objective To establish the quality standard of Qilong Huoluo Granules.Methods Thin-layer chromatography(TLC)method was used to qualitatively identify Cyathulae Radix,Astragali Radix,and Spatholobi Caulis in the preparation.High-performance liquid chromatography(HPLC)method was used to determine the content of salvianolic acid B in the preparation,the chromatographic column was Eclipse XDB-C18 column(150 mm×4.6 mm,5 μm),the mobile phase was acetonitrile-0.1%phosphoric acid solution(23 ∶ 77,V/V),the flow rate was 1.0 mL/min,the detection wavelength was 286 nm,the column temperature was 30℃,and the injection volume was 10 μL.Results The TLC spots were clear,and the negative control had no interference.The linear range of salvianolic acid B was 0.062 4-1.248 μg(r = 1.000,n = 6).The RSDs of precision,stability,and repeatability test results were all lower than 2.00%(n = 6).The average recovery of salvianolic acid B was 107.61%with an RSD of 4.28%(n = 6).The content of salvianolic acid B in five batches of samples was in the range of 1.09-1.32 mg/g.Conclusion This method is specific and accurate,which can be used for the quality control of Qilong Huoluo Granules.The content of salvianolic acid B in Qilong Huoluo Granules is temporarily set at no lower than 1.0 mg/g.
Qilong Huoluo GranulesTLCHPLCCyathulae RadixAstragali RadixSpatholobi Caulissalvianolic acid B
万方数据
维普
