Simultaneous Determination of 10 Components in Wuwei Xianglian Pills by RP-HPLC
Objective To establish a reverse-phase high-performance liquid chromatography(RP-HPLC)method for the simultaneous determination of 10 components in Wuwei Xianglian Pills,including berberine ydrochloride,coptisine,palmatine,tetrahydropalmatine,costunolide,dehydrocostuslactone,evodiamine,rutaecarpine,limonin,and paeoniflorin.Methods The chromatography column was Waters SunFire C18 column(250 mm×4 6 mm,5 μm),the mobile phase was 0.02 mol/L potassium dihydrogen phosphate solution containing 0.02％triethylamine(the pH was ajusted to 3.5 with phophoric acid)-acetonitrile(gradient elution),the flow rate was 1.0 mL/min,the column temperature was 35℃,the detection wavelength was 225 nm,and the injection volume was 10 μL.Results The linear ranges of berberine hydrochloride,coptisine,palmatine,tetrahydropalmatine,costunolide,dehydrocostuslactone,evodiamine,rutaecarpine,limonin and paeoniflorin were 6.852-51.39 μg/mL,2.956-22.17 μg/mL,2.918-21.88 μg/mL,4.643-34.82 μg/mL,4.871-36.53 μg/mL,4.007-30.05 μg/mL,4.519-33.89 μg/mL,4.482-33.62 μg/mL,2.724-20.43 μg/mL,and 2.809-21.07 μg/mL(r≥0.999 2,n = 6),respectively.The limits of quantification(LOQ)were 0.66,0.31,0.29,0.45,0.48,0.41,0.42,0.46,0.27,0.28 μg/mL,respectively.The RSDs of precision,stability,and repeatability test results were all lower than 2.0％(n = 6).The average recoveries of the above 10 components were 100.36％,98.74％,98.71％,99.67％,99.86％,99.59％,98.70％,98.38％,98.25％,98.72％,with RSDs of 0.89％,1.02％,1.04％,1.17％,0.84％,1.15％,1.33％,1.20％,1.07％,and 1.23％(n = 6),respectively.The average contents of the above 10 components in three batches of samples were 1.165-1.192 mg/g,0.523 1-0.527 6 mg/g,0.499 7-0.501 1 mg/g,0.681 4-0.688 9 mg/g,0.697 7-0.700 2 mg/g,0.606 8-0.613 4 mg/g,0.580 1-0.583 7 mg/g,0.427 7-0.442 2 mg/g,0.410 1-0.416 5 mg/g,and 0.400 2-0.401 3 mg/g(n = 2),respectively.Conclusion The established method is convenient and feasible,with accurate and reliable results and good stability,which can be used for the simultaneous determination of 10 components in Wuwei Xianglian Pills.
RP-HPLCWuwei Xianglian Pillscontent determination of multi-components
万方数据
维普
