Molecular Mechanism of Xiaojianzhong Decoction in the Treatment of Gastric Ulcer Based on Network Pharmacology
Objective To investigate the molecular mechanism of Xiaojianzhong Decoction in the treatment of gastric ulcer.Methods Based on the relevant studies in the CNKI and WanFang databases,the main active ingredients in traditional Chinese medicines in Xiaojianzhong Decoction were collected by the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP).The potential targets of Xiaojianzhong Decoction were collected by the TCMSP,PubChem,and Swiss TargetPrediction databases.The gastric ulcer-related targets were screened by the GeneCards,OMIM,Drugbank and Therapeutic Target Database(TTD).The Venn diagram was drawn to screen the common targets(key targets)of Xiaojianzhong Decoction and gastric ulcer.A network of drug-active ingredient-disease-target was constructed by the Cytoscape 3.7.1 software,and a protein-protein interaction(PPI)network for key targets was constructed by the String database.The Gene Ontology(GO)functional enrichment and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analyses were performed by the Metascape database.The key targets of maltose(principal drug)in the treatment of gastric ulcer were predicted.Results A total of 142 potential active ingredients,282 targets in the prescription,711 potential disease targets and 111 key targets were collected.The key targets with high degree included ALB,AKT1,TP53,IL-6,TNF,IL-1β,JUN and VEGFA.The GO functional enrichment analysis showed that 1 894 functions were obtained,among which there were 1 685 biological processes,133 molecular functions,and 76 cell compositions,mainly including positive regulation of cell motility,protein kinase binding,cytoplasmic vesicle lumen and so on.The KEGG pathway enrichment analysis showed that 178 signaling pathways were obtained,mainly including PI3K/Akt signaling pathway,MAPK signaling pathway and so on.Maltose(principal drug)promoted the ulcer healing mainly through the targets such as VEGFA and STAT3.Conclusion Xiaojianzhong Decoction treats gastric ulcer through multiple ingredients,multiple targets and multiple pathways.
万方数据
维普
