Objective To establish a quality standard for Shexiang Huayu Xingnao Granules.Methods The Moschus,Astragali Radix and Rhei Radix et Rhizoma in the preparation were identified qualitatively by the thin-layer chromatography(TLC)method.The content of ginsenoside Rb1 in the preparation was determined by high-performance liquid chromatography(HPLC)method;the chromatographic column was the UltimateTM C18 column(250 mm×4.6 mm,5 µm),the mobile phase was acetonitrile-water(gradient elution),the flow rate was 1.0 mL/min,the detection wavelength was 203 nm,the column temperature was 40℃,and the injection volume was 10 µL.Results The TLC chromatograms of Moschus,Astragali Radix and Rhei Radix et Rhizoma had clear spots and good resolution,and there was no interference from the negative reference.The linear range of ginsenoside Rb1 was 78.04-780.40 µg/mL(R2=0.999 1,n=6).The RSDs of precision,stability and repeatability tests were all lower than 3.0%.The average recovery rate of ginsenoside Rb1 was 96.84%with an RSD of 3.16%(n=9).Conclusion This method is simple,repeatable,accurate and reliable,which can be used for quality control of Shexiang Huayu Xingnao Granules.
关键词
麝香化瘀醒脑颗粒/薄层色谱法/高效液相色谱法/麝香/黄芪/大黄/人参皂苷Rb1
Key words
Shexiang Huayu Xingnao Granules/TLC/HPLC/Moschus/Astragali Radix/Rhei Radix et Rhizoma/ginsenoside Rb1
维普
万方数据