Study on Quality Standard of Shexiang Huayu Xingnao Granules
Objective To establish a quality standard for Shexiang Huayu Xingnao Granules.Methods The Moschus,Astragali Radix and Rhei Radix et Rhizoma in the preparation were identified qualitatively by the thin-layer chromatography(TLC)method.The content of ginsenoside Rb1 in the preparation was determined by high-performance liquid chromatography(HPLC)method;the chromatographic column was the UltimateTM C18 column(250 mm×4.6 mm,5 µm),the mobile phase was acetonitrile-water(gradient elution),the flow rate was 1.0 mL/min,the detection wavelength was 203 nm,the column temperature was 40℃,and the injection volume was 10 µL.Results The TLC chromatograms of Moschus,Astragali Radix and Rhei Radix et Rhizoma had clear spots and good resolution,and there was no interference from the negative reference.The linear range of ginsenoside Rb1 was 78.04-780.40 µg/mL(R2=0.999 1,n=6).The RSDs of precision,stability and repeatability tests were all lower than 3.0%.The average recovery rate of ginsenoside Rb1 was 96.84%with an RSD of 3.16%(n=9).Conclusion This method is simple,repeatable,accurate and reliable,which can be used for quality control of Shexiang Huayu Xingnao Granules.
Shexiang Huayu Xingnao GranulesTLCHPLCMoschusAstragali RadixRhei Radix et Rhizomaginsenoside Rb1
万方数据
维普
