Objective To improve the quality standard of Dashanzha Granules.Methods Crataegi Fructus in the samples was identified qualitatively by the thin-layer chromatography(TLC)method.The content of hyperoside in the samples was determined by the ultraviolet-visible spectrophotometry at a wavelength of 500 nm.Results The TLC chromatograms of Crataegi Fructus showed clear characteristic spots,good resolution,and there was no interference from the negative reference.The linear range of hyperoside was 0.004 9-0.049 0 mg/mL(R2=0.999 7,n=6).The RSDs of precision,stability and repeatability tests were all lower than 2.0%.The average recovery rate of hyperoside was 100.10%with an RSD of 0.64%(n=6).The average content of hyperoside in the samples was 38.26 mg per bag.Conclusion The established method is simple,accurate and reliable,which can be used for quality control of Dashanzha Granules.
关键词
大山楂颗粒/金丝桃苷/山楂/紫外-可见分光光度法/薄层色谱法/质量标准
Key words
Dashanzha Granules/hyperoside/Crataegi Fructus/ultraviolet-visible spectrophotometry/TLC/quality standard
维普
万方数据