Effect of miR-558 on Proliferation,Migration and Apoptosis of Lung Cancer Cells
Objective To investigate the effect of miR-558 on the proliferation,migration and apoptosis of lung cancer cells.Methods The cancer tissue and adjacent tissue specimens of 35 patients with lung cancer admitted to the hospital from May to August 2020 were collected.The human lung cancer cells A549 and human bronchial epithelial cells BEAS-2B in logarithmic growth phase were selected,to detect the expression levels of circ_0001017 and miR-558 by the real-time fluorescence quantitative polymerase chain reaction(qPCR)method.The A549 cells in logarithmic growth phase were selected for transfection and divided into the empty plasmid(pcDNA)group(group A),pcDNA-circ_0001017 group(group B),pcDNA-circ_0001017+miR-NC group(group C),and pcDNA-circ_0001017+miR-558 group(group D).The dual-luciferase reporter assay was used to detect the targeting relationship between circ_0001017 and miR-558.The MTT assay,plate clone formation assay,Transwell assay and flow cytometry were used to detect the cell activity,cloning,migration,and apoptosis,respectively.The qPCR method was used to detect the expression levels of circ_0001017 and miR-558.The Western blot was used to detect the expression levels of B-cell lymphoma-2 protein(Bcl-2)and Bcl-2-associated X protein(Bax).Results Compared with those in the adjacent tissues,the expression level of circ_0001017 in the lung cancer tissue significantly decreased(P<0.05),while the expression level of miR-558 significantly increased(P<0.05).Compared with those in the BEAS-2B cells,the expression level of circ_0001017 in A549 cells significantly decreased(P<0.05),while the expression level of miR-558 significantly increased(P<0.05).Overexpression of miR-558 could significantly decrease the luciferase activity of wild-type vector(WT)-circ_0001017(P<0.05).Compared with those in group A,the proliferation inhibition rate,the apoptosis rate,and the expression levels of circ_0001017 and Bax in group B significantly increased(P<0.05),while the number of cell clone formation and cell migration significantly decreased(P<0.05),and the expression levels of miR-558 and Bcl-2 significantly decreased(P<0.05).Compared with those in group C,the proliferation inhibition rate,the apoptosis rate,and the expression levels of circ_0001017 and Bax in group D significantly decreased(P<0.05),while the bumber of cell clone formation and cell migration significantly increased(P<0.05),and the expression levels of Bcl-2 and miR-558 significantly increased(P<0.05).Conclusion Overexpression of miR-558 can weaken the effect of overexpression of circ_0001017 on the inhibition of the proliferation,clone formation,migration,and promote cell apoptosis.
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