Effect of Triptolide on Proliferation,Invasion and Migration of MGC803 Cells Through the AKT/GSK-3β Pathway
Objective To investigate the effect of triptolide on the proliferation,invasion,and migration of MGC803 cells through the serine threonine protein kinase/glycogen synthase kinase 3(AKT/GSK-3β)pathway.Methods MGC803 cells were cultured in vitro to determine the intervention dose and intervention time of triptolide.The cells were divided into the negative control group(group A,without treatment),positive control group(group B,10 mol/L cisplatin),and triptolide high-,medium-,and low-dose groups(groups C,D,and E).After 48 h of intervention,Transwell assay was used to detect cell invasion ability,scratch assay was used to detect migration ability,and Western blot was used to detect the expression of related proteins.Results With the concentration of triptolide increasing,the proliferation rate of MGC803 cells decreased(P<0.05).The IC50 was the lowest at 48 h,so 48 h was selected as the intervention time.The intervention doses for groups C,D,and E were 80,40,and 20 nmol/L,respectively.Compared with those in group B,the number of the invasive cells,the cell migration distance,and the expression levels of vimentin,snail,p-AKT/AKT,and p-GSK-3β/GSK-3β all significantly increased(P<0.05),while the expression levels of E-cadherin and β-catenin significantly decreased in groups C,D,and E(P<0.05),which showed a dose-dependent relationship.Conclusion Triptolide can inhibit MGC803 cell proliferation,invasion,and migration by inhibiting AKT/GSK-3β signal pathways.
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