Chemical Constituent Analysis of Qingre Liangxue Formula Based on UPLC-Q Exactive Focus MS/MS
Objective To study the chemical constituents of Qingre Liangxue Formula.Methods Studies on the chemical constituents of relevant medicinal herbs of Qingre Liangxue Formula in the CNKI,PubChem and other databases were searched to establish a chemical constituent database.The spectral data of the formula were analyzed by the ultra-high-performance liquid chromatography quadrupole/electrostatic field orbitrap high-resolution mass spectrometry(UPLC-Q Exactive Focus MS/MS)method,the molecular formulas were fitted by the Xcalibur 4.0 software and matched with the information of the self-built database to preliminarily infer the molecular formula information.On this basis,the target chemical constituents were identified and confirmed according to relevant chemical constituent data and study information.Results Sixty-six chemical constituents were identified,including twenty monoterpenoid glycosides,ten phenolic acids,eight iridoid glycosides,four glycosides,four galloylglucoses,two organic acids,two phenylethanoid glycosides,two coumarins,one phenylpropanoid,one alkaloid,one flavone,one cyclic peptide,one naphthoquinone,one furfural and eight other constituents.Conclusion The established method can systematically and qualitatively analyze the chemical constituents in the Qingre Liangxue Formula.
国家科技期刊平台
NSTL
万方数据
