Content Determination of Paeoniflorin in Compound Huangfan Oral Liquid by HPLC
Objective To establish a high-performance liquid chromatography(HPLC)method for the content determination of paeoniflorinof in Compound Huangfan Oral Liquid.Methods The chromatography column was Agilent Eclipse Plus C18 column(150 mm×4 6 mm,5 μm),the mobile phase was methanol-0.1% phosphoric acid solution(26∶74,V/V),the flow rate was 1.0 mL/min,the detection wavelength was 230 nm,the column temperature was 30℃,and the injection volume was 10 μL.Results The linear range of paeoniflorin was 15.35-168.85 μg/mL(r=0.999 6,n=6).The RSDs of precision,stability,and repeatability test results were all lower than 1.0% (n=6).The average recovery of paeoniflorin was 95.03% with an RSD of 0.78% (n=9).Conclusion The method is accurate,stable,and has good repeatability,which can be used for the content determination of paeoniflorin in Compound Huangfan Oral Liquid.
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