Content Determination of Vitamin C in Enteral Nutritional Suspension by HPLC
Objective To establish a high-performance liquid chromatography(HPLC)method for the content determination of vitamin C in Enteral Nutrition Suspension.Methods The chromatography column was Acutfex YS-NH2 column(250 mm×4.6 mm,5 μm),the mobile phase was methanol-1% citric acid aqueous solution(80∶20,V/V),the flow rate was 1.0 mL/min,the detection wavelength was 254 nm,the column temperature was 30℃,and the injection volume was 10 μL.Results The linear range of vitamin C was 1.01-20.16 μg/mL(r=0.999 5,n=6),the limit of detection was 0.13 ng/mL,and the limit of quantification was 0.42 ng/mL.The RSDs of precision,stability,and repeatability test results were all lower than 2.0% (n=6).The average recovery of vitamin C was 98.15% with an RSD of 1.34% (n=9).The content of vitamin C in five batches of samples was in the range of 120.18-233.95 μg/mL.Conclusion The method is simple,accurate,and highly sensitive,which can be used for the content determination of vitamin C in Enteral Nutrition Suspensions.
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