高效液相色谱法测定肠内营养混悬液中维生素C含量
Content Determination of Vitamin C in Enteral Nutritional Suspension by HPLC
金舒 1申兰慧1
作者信息
- 1. 江苏省无锡市药品安全检验检测中心,江苏 无锡 214028
- 折叠
摘要
目的 建立测定肠内营养混悬液中维生素C含量的高效液相色谱法.方法 色谱柱为Acutfex YS-NH2 柱(250 mm×4.6 mm,5μm),流动相为甲醇-1%枸橼酸水溶液(80∶20,V/V),流速为 1.0 mL/min,检测波长为 254 nm,柱温为 30℃,进样量为 10 μL.结果 维生素C的质量浓度在 1.01～20.16 μg/mL范围内与峰面积线性关系良好(r=0.999 5,n=6);检测限为 0.13 ng/mL,定量限为 0.42 ng/mL;精密度、稳定性、重复性试验结果的RSD均小于 2.0%(n=6);平均回收率为 98.15%,RSD为 1.34%(n=9).5 批样品中维生素C的含量为 120.18～233.95 μg/mL.结论 该方法操作简便、结果准确、灵敏度高,可用于肠内营养混悬液中维生素C的含量测定.
Abstract
Objective To establish a high-performance liquid chromatography(HPLC)method for the content determination of vitamin C in Enteral Nutrition Suspension.Methods The chromatography column was Acutfex YS-NH2 column(250 mm×4.6 mm,5 μm),the mobile phase was methanol-1% citric acid aqueous solution(80∶20,V/V),the flow rate was 1.0 mL/min,the detection wavelength was 254 nm,the column temperature was 30℃,and the injection volume was 10 μL.Results The linear range of vitamin C was 1.01-20.16 μg/mL(r=0.999 5,n=6),the limit of detection was 0.13 ng/mL,and the limit of quantification was 0.42 ng/mL.The RSDs of precision,stability,and repeatability test results were all lower than 2.0% (n=6).The average recovery of vitamin C was 98.15% with an RSD of 1.34% (n=9).The content of vitamin C in five batches of samples was in the range of 120.18-233.95 μg/mL.Conclusion The method is simple,accurate,and highly sensitive,which can be used for the content determination of vitamin C in Enteral Nutrition Suspensions.
关键词
高效液相色谱法/肠内营养混悬液/维生素C/含量测定Key words
HPLC/Enteral Nutrition Suspension/vitamin C/content determination引用本文复制引用
基金项目
江苏省市场监督管理局科技计划项目(KJ2022029)
出版年
2024
国家科技期刊平台
NSTL
万方数据