Inhibitory Effect of Edaravone on the Inflammatory Reactions of Mouse Leukemic Monocyte Macrophage RAW264.7 Cells
Objective To investigate the inhibitory effect of edaravone(EDA)on the inflammatory reactions of mouse leukemic monocyte macrophage RAW264.7 cells.Methods RAW264.7 cells were induced by lipopolysaccharide to replicate the inflammatory cell model.The CCK-8 method was used to detect the cytotoxic effect of EDA.The RAW264.7 cells were divided into the blank group(equal volume of medium),model group(equal volume of medium),and drug group(40 µg/mL EDA).The Griess method was used to detect the nitric oxide(NO)level in cells,the enzyme-linked immunosorbent assay(ELISA)was used to detect the prostaglandin E2(PGE2)and interleukin-1β(IL-1β),interleukin-18(IL-18),tumor necrosis factor-α(TNF-α)and reactive oxygen species(ROS)levels.The real-time fluorescence quantitative polymerase chain reaction(qPCR)was used to detect the expression levels of IL-1β and IL-18 mRNA in cells.The Western blot was used to detect the expression levels of JAK2/signal transducer and activator of transcription 3(STAT3)pathway-related proteins including JAK2,p-JAK2,STAT3,p-STAT3,IL-1β and IL-18.Results Compared with that at 0 µg/mL,there was no significant change in survival rate of cells at 20,40,80,160 µg/mL EDA(P<0.05).Compared with those in the model group,the NO,PGE2,IL-1β,IL-18,TNF-α and ROS levels in the drug group were significantly lower(P<0.05);the expression levels of IL-1β,IL-18 mRNA and proteins,p-JAK2/JAK2 and p-STAT3/STAT3 in the drug group were significantly lower(P<0.05).Conclusion EDA may inhibit the inflammatory reactions of RAW264.7 cells by inhibiting the activation of the JAK2/STAT3 pathway.
国家科技期刊平台
NSTL
万方数据
