Improvement of Quality Standard of Xinmaishu Yihao Oral Liquid
Objective To improve the quality standard of Xinmaishu Yihao Oral Liquid.Methods Ophiopogonis Radix and Schisandrae Chinensis Fructus in the preparation were qualitatively identified by the thin layer chromatography(TLC)method.With rutin and D(+)-anhydrous glucose as the reference,the contents of total flavonoids and total polysaccharides in the preparation were determined by the ultraviolet-visible spectroscopy(UV-Vis)at wavelengths of 504,490 nm,respectively.Results The TLC chromatograms of Ophiopogonis Radix and Schisandrae Chinensis Fructus showed clear spots,good resolution,and there was no interference from the negative reference.The linear ranges of rutin and D(+)-anhydrous glucose were 0.016 7-0.058 5 mg/mL(R2=0.999 3,n=6),0.002 7-0.016 2 mg/mL(R2=0.987 9,n=6)respectively.The RSDs of precision,stability and repeatability tests were all lower than 1.0% .The average recovery rates of total flavonoids and total polysaccharides were 99.85% and 99.80%,with the RSDs of 0.04% and 0.22% (n=6)respectively.The average contents of total flavonoids and total polysaccharides in 19 batches of preparation were 0.695,22.30 mg/mL respectively.Conclusion This method is easy,sensitive,precise,stable and repeatable,which can be used for the quality control of Xinmaishu Yihao Oral Liquid.
Xinmaishu Yihao Oral LiquidTLCUV-Vistotal flavonoidstotal polysaccharidesquality control
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