HPLC Characteristic Chromatograms of Torreyae Semen Tostum
Objective To establish a high-performance liquid chromatography(HPLC)characteristic chromatograms of Torreyae Semen Tostum.Methods The chromatographic column was the Thermo Hypersil Gold C18 column(250 mm×4.6 mm,5 µm),the mobile phase was acetonitrile-0.1% phosphoric acid aqueous solution(gradient elution),the flow rate was 1.0 mL/min,the detection wavelength was 203 nm,the column temperature was 30℃,and the injection volume was in the range of 10 µL.The HPLC characteristic chromatograms of 13 batches of medicinal material decoction pieces were established,the Similarity Evaluation Software for Chromatographic Fingerprint of Traditional Chinese Medicine(2012 Version)was used for similarity evaluation,the characteristic peaks were marked and the common peaks were identified.Results Seven characteristic peaks were marked,the RSDs of relative retention time were lower than 1%,and the RSDs of relative peak area were in the range of 7.31%-70.45% .Two of them were identified,namely linoleic acid and oleic acid.The similarity of 13 batches of medicinal material decoction pieces was in the range of 0.827-0.990,with only two batches below 0.900.Conclusion This method is simple,feasible,stable and accurate,which can help to improve the processing specifications and quality standards of Torreyae Semen Tostum Decoction Pieces in various producing areas.
Torreyae Semen TostumHPLCcharacteristic chromatogramproducing areaprocessingquality control
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