Determination of Five Harmful Elements in Ten Famous Herbal Drugs in Shanxi by ICP-MS and Risk Assessment
Objective To establish an inductively coupled plasma mass spectrometry(ICP-MS)method for the content determination of five harmful elements[lead(Pb),arsenic(As),cadmium(Cd),mercury(Hg),and copper(Cu)]in ten famous herbal drugs in Shanxi,such as Astragali Radix,Codonopsis Radix,Forsythiae Fructus,Polygalae Radix,Bupleuri Radix,Scutellariae Radix,Ziziphi Spinosae Semen,Sophorae Flavescentis Radix,Crataegi Fructus,Persicae Semen.Methods The samples were pre-treated by microwave digestion method,with germanium(72Ge),indium(115In),and bismuth(209Bi)as internal standards.The ICP-MS method was adopted with the radio frequency(RF)power of 1 200 W,the collision mode was adopted,the collision gas was helium,the carrier gas was argon,the carrier gas flow rate was 0.70 L/min,the plasma gas flow rate was 9.00 L/min,and the pump speed was 0.3 r/s.Results The linear ranges of Pb,As,Cd,Hg,and Cu were 0-50 ng/mL,0-50 ng/mL,0-10 ng/mL,0-5ng/mL,and0-200ng/mL(R2≥0.999 4),respectively.The limits of detection(LOD)were 0.20,0.20,0.02,0.01,and 0.50 ng/mL,respectively.The limits of quantification(LOQ)were 0.70,0.70,0.06,0.03,and 1.70 ng/mL,respectively.The recovery rates of Pb,As,Cd,Hg,and Cu were 94.94%,81.30%,88.30%,85.00%,and 93.81%,with RSDs of 2.01%,1.63%,1.32%,1.83%,and 1.12%(n=6),respectively.The RSD of the repeatability test result was in the range of 0.92%-2.31%(n=6).The RSD of the precision test result was in the range of 0.81%-3.81%(n=6).The target hazard quotients(THQ)for risk assessment of harmful elements such as Pb,As,Cd,Hg,and Cu were all lower than 1.Conclusion The method is simple and sensitive,which can provide a reference for the establishment and evaluation of analysis methods for harmful elements in the ten famous herbal drugs in Shanxi.
ICP-MSten famous herbal drugs in Shanxiharmful elementsrisk assessment
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