Objective To establish the quality standard of Yangwei Granules.Methods Astragali Radix,Corydalis Rhizoma,Clematidis Radix et Rhizoma,Forsythiae Fructus,Aurantii Immaturus Fructus,and Glycyrrhizae Radix et Rhizoma were identified by the thin-layer chromatography(TLC)method.The content of paeoniflorin was determined by the high-performance liquid chromatography(HPLC)method,the chromatographic column was Dikma Diamonsil C18 column(250 mm×4.6 mm,5 µm),the mobile phase consisted of a mixed solution of acetonitrile-0.1%phosphoric acid solution(gradient elution),the flow rate was 1.0 mL/min,the column temperature was 30℃,the detection wavelength was 230 nm,and the injection volume was 10 µL.Results The TLC chromatograms had good separation,with clear color of characteristic spots,and no interference from negative samples.The chromatographic peak of paeoniflorin was completely separated with a retention time of 12.204 min.The linear range of paeoniflorin was 5-100 µg/mL(r=0.999 7,n=6),and the RSDs of precision,stability,and repeatability test results were all lower than 2.0%.The average recovery of paeoniflorin was 98.16%with an RSD of 0.67%(n=6).Conclusion The established methods are simple,sensitive,highly specific,and reproducible,which can be used for the quality control of Yangwei Granules.
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