Study on Quality Standard of Shengui Yixue Mixture
Objective To establish the quality standard of Shengui Yixue Mixture.Methods Polygoni Cuspidati Rhizoma et Radix,Angelicae Sinensis Radix and Paeoniae Radix Alba in the preparation were qualitatively identified by the thin-layer chromatography(TLC)method.The content of paeoniflorin was determined by the high-performance liquid chromatography(HPLC)method;the chromatographic column was the Hypersil GOLD C18 column(250 mm×4.6 mm,5 μm),the mobile phase was acetonitrile-0.1%phosphoric acid aqueous solution(gradient elution),the flow rate was 1.0 mL/min,the detection wavelength was 230 nm,the column temperature was 30℃,and the injection volume was 5 μL.Results The TLC chromatograms of the three medicinal herbs showed clear characteristic spots,good resolution,and there was no interference from the negative reference.The linear range of paeoniflorin was 0.00732-0.1464 mg/mL(R2=0.9998,n=5).The RSDs of precision,stability and repeatability tests were all lower than 1.0%.The average recovery rate of paeoniflorin was 101.92%with an RSD of 0.66%(n=6).The contents of paeoniflorin in three batches of samples were 0.2640,0.2819,0.2716 mg/mL,respectively.Conclusion The established method is easy,accurate and reliable,which can be used for quality control of Shengui Yixue Mixture.