Objective To establish the quality standard of Shengui Yixue Mixture.Methods Polygoni Cuspidati Rhizoma et Radix,Angelicae Sinensis Radix and Paeoniae Radix Alba in the preparation were qualitatively identified by the thin-layer chromatography(TLC)method.The content of paeoniflorin was determined by the high-performance liquid chromatography(HPLC)method;the chromatographic column was the Hypersil GOLD C18 column(250 mm×4.6 mm,5 μm),the mobile phase was acetonitrile-0.1%phosphoric acid aqueous solution(gradient elution),the flow rate was 1.0 mL/min,the detection wavelength was 230 nm,the column temperature was 30℃,and the injection volume was 5 μL.Results The TLC chromatograms of the three medicinal herbs showed clear characteristic spots,good resolution,and there was no interference from the negative reference.The linear range of paeoniflorin was 0.00732-0.1464 mg/mL(R2=0.9998,n=5).The RSDs of precision,stability and repeatability tests were all lower than 1.0%.The average recovery rate of paeoniflorin was 101.92%with an RSD of 0.66%(n=6).The contents of paeoniflorin in three batches of samples were 0.2640,0.2819,0.2716 mg/mL,respectively.Conclusion The established method is easy,accurate and reliable,which can be used for quality control of Shengui Yixue Mixture.
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