Improvement of Quality Standard of Zhituojing Granules
Objective To establish a quality standard of Zhituojing Granules.Methods Polygoni Multiflori Radix Praeparata,Ligustri Lucidi Fructus,Poria and Chaenomelis Fructus in the preparation were qualitatively identified by the thin-layer chromatography(TLC)method.The content of paeoniflorin in the preparation was determined by the high-performance liquid chromatography(HPLC)method;the chromatographic column was the Agilent Hedera Lichrospher column(250 mm×4.6 mm,5 μm),the mobile phase was acetonitrile-0.1%phosphoric acid aqueous solution(18∶82,V/V),the flow rate was 1.0 mL/min,the detection wavelength was 230 nm,the column temperature was 35℃,and the injection volume was 10 μL.Results The TLC chromatograms of Polygoni Multiflori Radix Praeparata,Ligustri Lucidi Fructus,Poria and Chaenomelis Fructus showed clear spots,good resolution,and there was no interference from the negative reference.The linear range of paeoniflorin was 21-168 μg/mL(r=0.9995,n=5).The RSDs of precision,stability and repeatability tests were all lower than 3.0%.The average recovery rate of paeoniflorin was 100.64%with an RSD of 1.64%(n=9).Conclusion This method is easy,accurate and reliable,which can be used for the quality control of Zhituojing Granules.