Quality Evaluation of Pinelliae Rhizoma and Its Processed Products Before and After Sulfur Fumigation Based on UPLC-Q-TOF-MS
Objective To establish an ultra-performance liquid chromatography-quadrupole/time-of-flight high-resolution mass spectrometry(UPLC-Q-TOF-MS)method for evaluating the quality of Pinelliae Rhizoma and its processed products before and after sulfur fumigation.Methods The chromatographic column was Waters HSS T3 column(100 mm × 2.1 mm,1.7 μm),the mobile phase was methanol acetonitrile(1∶1,V/V)-0.01 formic acid solution(gradient elution).Compound data of Pinelliae Rhizoma and its processed products before and after sulfur fumigation were collected under positive and negative ion monitoring modes,and those were compared with compounds retrieved from Chemspider,mzCloud,Metaboanalyst databases,and relevant literature.The Sciex OS software was used to analyze the collected data of the sample processed by sulfur fumigation,identify characteristic neutral loss or characteristic fragment ions of sulfur-containing compounds,and determine the sulfur-containing products of Pinelliae Rhizoma and its processed products after sulfur fumigation.The Pinelliae Rhizoma and its processed products were classified by the partial least squares-discriminant analysis(PLS-DA)method.Results A total of 254 compounds were identified from Pinelliae Rhizoma and its processed products,and the main compounds identified were small molecule compounds such as amino acids,nucleosides and their derivatives,organic acids,and natural products such as saponins,alkaloids and lignin derivatives.A total of 31 sulfur-containing compounds could be detected in all the processed products of Pinelliae Rhizoma(Pinelliae Rhizoma Praeparatum cum Zingibere et Alumine,Pinelliae Rhizoma Praeparatum,and Pinelliae Rhizoma Praeparatum cum Alumine)after sulfur fumigation,among which 6-gingerol was found only in Pinelliae Rhizoma Praeparatum cum Zingibere et Alumine,Daidzein and glycyrrhizin were found only in Pinelliae Rhizoma Praeparatum.In Pinelliae Rhizoma processed with different degrees of sulfur fumigation,the trend of peak area changes in sulfur content was basically in line with the trend of severe sulfur fumigation,exceeding the limit＞slightly fumigation,and slightly exceeding the limit＞no fumigation.Conclusion The established UPLC-Q-TOF-MS method can rapidly and comprehensively analyze the chemical compounds of Pinelliae Rhizoma and its processed products.Sulfur fumigation can affect the quality of Pinelliae Rhizoma and its processed products.During the processing of Pinelliae Rhizoma,sulfur fumigation technology should be used in a reasonable and standardized manner to avoid the impact of excessive sulfur fumigation on the quality of Pinelliae Rhizoma.
UPLC-Q-TOF-MSPinelliae Rhizomaprocessed products of Pinelliae Rhizomasulfur fumigationchemical compoundsquality evaluation
万方数据
维普
