Diagnostic Value and Regulatory Mechanism of Differentially Expressed miR in Endometriosis
Objective To investigate the diagnostic value of differentially expressed microRNA(miR)in endometriosis(EMT),and to analyze its regulatory mechanism.Methods A total of 75 patients with EMT admitted to the Obstetrics and Gynecology Department of the Affiliated Suzhou Hospital of Nanjing Medical University from March 2022 to March 2023 were selected as the EMT group,and 75 healthy volunteers were selected as the normal control group during the same period.The miR chip analysis technology was used to screen the differentially expressed miRNAs in the blood samples of the research subjects.Quantitative polymerase chain reaction(qPCR)was used to detect the expression levels of miR-410-3p in the blood samples of 75 EMT patients.Of which,blood samples of 60 EMT patients who were scheduled for surgery on the day before surgery and two months after surgery were also verified.The receiver operating characteristic(ROC)curve was used to analyze the diagnostic value of miR-410-3p in EMT.Bioinformatics analysis was carried out through the TargetScan database to screen target genes.Dual luciferase reporter gene assay and Western blot were used to detect the expression levels of transfection of miR-410-3p and the vascular endothelial growth factor A(VEGFA).Results A total of 87 differentially expressed miRs were screened out.Compared with those in the normal control group,the expression of miR-365 was significantly up-regulated in the EMT group(P＜0.05),while the expression of miR-410-3p was significantly down-regulated in the EMT group(P＜0.05).The area under the ROC curve(AUC)of miR-410-3p was 0.770 5.The verification test of preoperative and postoperative blood samples of 60 EMT patients who were scheduled for surgery showed that the expression of miR-410-3p was significantly up-regulated after lesion tissue resection(P＜0.05).After transfection with miR-410-3p,the expression levels of VEGFA was significantly down-regulated(P＜0.05).Conclusion Plasma miR-410-3p can target the regulation of VEGFA expression as a biomarker for EMT patients,and it has good diagnostic potential,which may exert regulatory effects by targeting the inhibition of VEGFA.
万方数据
维普
