Molecular Mechanism of Xinmaishuyihao Oral Liquid in the Treatment of Heart Disease Based on Network Pharmacology
Objective To investigate the molecular mechanism of Xinmaishuyihao Oral Liquid in the treatment of heart disease.Methods The active ingredients of 11 traditional Chinese medicines(including Rehmanniae Radix,Asparagus Radix,Ophiopogonis Radix,Ziziphi Sujuba Semen,Platycladi Semen,Salviae Miltiorrhizae Radix,Angelicae Sinensis Radix,Pseudostellariae Radix,Cocos Poria,Paeoniae Alba Radix,and Schisandrae Chinensis Fructus)were obtained through the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP).Protein target prediction was performed.The disease targets related to heart disease were obtained from the GeneCards database,and the Venny 2.1.0 software was used to screen the common targets between the active components of Xinmaishuyihao Oral Liquid and heart disease.The Cytoscape 3.9.1 software was used to construct the network diagram of Xinmaishuyihao Oral Liquid-active components-predicted targets and the network diagram of active components-disease targets,and their core components were analyzed.String 11.0 database was used to construct the protein-protein interaction(PP1)network,and Cytoscape 3.9.1 software was used to screen the key and core targets.Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analysis and Gene Ontology(GO)functional analysis were performed by the Metascape database.The core components and core targets were verified by molecular docking.Results A total of 169 active components and 37 key targets of active components-disease were collected.A total of 1 097 disease targets with 197 intersecting targets were collected.The core components were quercetin,luteolin,ursolic acid,etc.The core targets were PTGS2,SCN5A,SLC6A2,etc.A total of 17 KEGG signaling pathways were obtained,mainly involving proteoglycans in cancer,human cytomegalovirus infection,AGE-RAGE signaling pathway,etc.A total of 1 205 GO functional items were obtained,including 944 biological processes(BP),146 molecular functions(MF),and 115 cellular components(CC),mainly involving endothelium migration,aging,inflammatory response,positive regulation of transcription-DNA templating,negative regulation of gene expression,positive regulation of cell proliferation,etc.The molecular docking results showed that the core components had good binding activity with their core targets.Conclusion Xinmaishuyihao Oral Liquid can treat heart disease through multiple components,targets,and pathways.
万方数据
维普
