Effects of Regulation of miRNA-4465 Targeting Wnt5A on the Proliferation,Migration and Invasion of Glioma Cells
Objective To investigate the effects of miRNA-4465(miR-4465)on the proliferation,invasion,and migration of glioma cells and relevant mechanism.Methods The U118 and U87 cells were treated by the miR-4465 mimic to detect the cell biological activity.The binding of miR-4465 and Wnt5A was evaluated by the dual-luciferase reporter gene test.The expression of Wnt5A and miR-4465 was inhibited by the siRNA and miR-4465 inhibitors,respectively.The proliferation,migration and invasion abilities of cells were detected by the CCK-8 method,clone formation assay,wound healing assay and Transwell assay.The effects of overexpression and inhibition of miR-4465 on tumor growth were evaluated by constructing transplanted-tumor nude mice model.Results The miR-4465 mimic significantly inhibited the proliferation ability of U118 and U87 cells.The dual-luciferase reporter gene test showed a targeted regulatory effect between miR-4465 and Wnt5A.Wnt5A silence could decrease the proliferation,migration and invasion abilities of cells,the above abilities of cells significantly increased after miR-4465 inhibition(P<0.05).In addition,Wnt5A silence could reversed the increased cell proliferation,migration and invasion abilities induced by miR-4465 inhibition.The in vivo tests showed that overexpression of miR-4465 inhibited the tumor growth and ki67 expression,while the inhibition of miR-4465 promoted tumor growth and ki67 expression(P<0.05).Conclusion The expression of miR-4465 is low in gliomas,and its overexpression can inhibit the proliferation,migration and invasion abilities of glioma cells.Its downstream mechanism is related to the Wnt5A pathway.
万方数据
维普
