摘要
目的 比较 3 种形态西洋参 6 种人参皂苷在大鼠体内的吸收情况.方法 以西洋参传统饮片、粗粉、破壁饮片中的 6 种人参皂苷(人参皂苷Re、Rb1、Rc、Rb2、Rb3、Rd)为考察指标.采用高效液相色谱-电喷雾检测器(HPLC-CAD)法,测定大鼠血清中 6 种人参皂苷的血药浓度,采用DAS 2.0 软件进行拟合,得到药物代谢动力学参数.比较 3 种形态西洋参 6 种人参皂苷在大鼠体内的吸收差异.结果 与传统饮片和粗粉比较,西洋参破壁饮片 6 种人参皂苷的峰浓度及药-时曲线下面积更优(P<0.05),且半衰期更长、达峰时间更短、生物利用度更高,综合排序为破壁饮片>粗粉>传统饮片.结论 与传统饮片及粗粉比较,等剂量下西洋参破壁饮片中 6 种人参皂苷在大鼠体内消除速率更慢,相对生物利用度更高.
Abstract
Objective To compare the absorption of six ginsenosides in three forms of Panacis Quinquefolii Radix in rats.Methods With six ginsenosides(ginsenosides Re,Rb1,Rc,Rb2,Rb3,Rd)in traditional decoction pieces,crude powder,and wall-breaking decoction pieces of Panacis Quinquefolii Radix as the investigation indicators,the blood concentrations of six ginsenosides in rat serum were determined by the high-performance liquid chromatography-charged aerosol detector(HPLC-CAD)method.The pharmacokinetic parameters were obtained by fitting with the DAS 2.0 software.The absorption differences of six ginsenosides in three forms of Panacis Quinquefolii Radix in rats were compared.Results Compared with those in the traditional decoction pieces and crude powder,the peak concentration and area under the blood concentration-time curve of the six ginsenosides in the wall-breaking decoction pieces of Panacis Quinquefolii Radix were better(P<0.05),the half-life was longer,the time to peak was shorter,and the bioavailability was higher.The comprehensive evaluation of wall-breaking decoction pieces was the best,followed by the crude powder and traditional decoction pieces.Conclusion Compared with those in the traditional decoction pieces and crude powder,the elimination rate of six ginsenosides in the wall-breaking decoction pieces of Panacis Quinquefolii Radix at the equal dose in rats is lower and the relative bioavailability is higher.
基金项目
安徽省重点研究和开发计划项目(201904a07020076)
维普
万方数据