Effect of Water Extract of Galla Turcica on the Biological Activity of Human Periodontal Ligament Fibroblasts
Objective To investigate the role of water extract of Galla Turcica in the prevention and treatment of oral inflammatory diseases.Methods The human periodontal ligament fibroblasts(HPDLFs)were isolated and cultured,and divided into the negative control group(PBS),the lipopoly saccharide group(1 µg/mL),the Galla Turcica group(10 mg/mL),and chlorhexidine group(0.2%,m/m).The gross examination was performed on the water extract powder of Galla Turcica,the scanning electron microscope(SEM)was used to observe the microscopic characteristics of the the above water extract powder.The MTT assay was used to measure cell viability,the XTT assay was used to detect cell proliferation level,the wound healing assay was used to investigate cell migration,and the enzyme-linked immunosorbent assay(ELISA)was used to detect the expression levels of interleukin-1β(IL-1β),interleukin-6(IL-6),and tumor necrosis factor-α(TNF-α).Results The gross examination and characteristics microscopic characteristics of water extract powder of Galla Turcica were obvious.The cell viability in the Galla Turcica group was significantly higher than that in the other groups(P<0.05).On the 3rd,5th,and 7th days after administration,the proliferation level of HPDLFs in the Galla Turcica group was significantly higher than that in the other groups(P<0.05).Compared with those in the negative control group,the expression levels of IL-1β,IL-6 and TNF-α in the Galla Turcica group significantly decreased(P<0.05).The wound healing assay after 24 h showed that the scratch wound in the Galla Turcica group almost completely healed.Conclusion The water extract of Galla Turcica can relieve the inflammatory reactions of HPDLFs.
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