Establishment of HPLC Fingerprint of Lanqin Granules and Content Determination of Nine Indicator Components
Objective To establish a high-performance liquid chromatography(HPLC)fingerprint of Lanqin Granules,and to determine the contents of nine indicator components.Methods The chromatographic column was the Agilent Infinity Lab Poroshell 120 EC-C18 column(150 mm×4.6 mm,2.7 µm),the mobile phase was acetonitrile-0.02 mol/L sodium dihydrogen phosphate solution(pH 4.2)with gradient elution,the flow rate was 1.0 mL/min,the detection wavelength was 230 nm,the column temperature was 30℃,and the injection volume was 10 µL.The characteristic HPLC fingerprint of 12 batches of samples was established by the Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine(2012 Version),and the similarity was calculated;the common peaks were marked and identified,and the contents of identified components were determined.Results There were 23 common peaks in the HPLC fingerprint of Lanqin Granules,with a similarity greater than 0.92.Nine of them were identified,including(R,S)-goitrin,genipin 1-gentiobioside,geniposide,phellodendrine chloride,baicalin,oroxyloside,wogonoside,berberine hydrochloride,wogonin.The contents of the above nine components were 0.026 2-7.815 µg/mL,0.204 0-411.600 µg/mL,0.122 2-377.400 µg/mL,0.195 1-15.360 µg/mL,0.078 7-297.000 µg/mL,0.092 0-410.103 µg/mL,0.201 9-149.625 µg/mL,0.101 8-7.538 µg/mL,0.101 9-19.200 µg/mL(r≥0.999 8),respectively.The limits of detection(LOD)were in the range of 0.006 5-0.051 1 µg/mL,and the limits of quantification(LOQ)were in the range of 0.026 2-0.204 0 µg/mL.The RSDs of the intermediate precision,stability and repeatability tests were all lower than 3.0%.The average recovery rates of the above nine components were in the range of 96.37%-101.69%,with the RSDs in the range of 0.48%-0.91%(n=6).Conclusion This method is sensitive,simple,and accurate,which can be used for the quality control of Lanqin Granules.
万方数据
维普
