Determination of meso-Dihydroguaiaretic Acid in Kadsura Coccinea Root by HPLC
Objective To establish a high-performance liquid chromatography method for determining the content of meso-dihydroguaiaretic acid(meso-DGA)in the ethyl acetate extract(ET)and anti-inflammatory active fraction ET5 of Kadsura coccinea root.Methods The chromatographic column was the BDS Hypersil C18 column(150 mm×4.6 mm,5 µm),the mobile phase was 0.1%formic acid aqueous solution-acetonitrile(gradient elution),the flow rate was 1.0 mL/min,the detection wavelength was 282 nm,the column temperature was 45℃,the injection volume was 10 µL.Results The linear range of meso-DGA was 1.0-50.0 µg/mL(R2=0.999 4,n=6).The RSDs of precision,stability and repeatability tests were not greater than 2.0%.The average recovery rate of meso-DGA was 100.08%,with an RSD of 1.02%(n=6).Conclusion The established method is easy,repeatable and accurate,which can be used to determine the content of meso-DGA in ET and ET5 of Kadsura coccinea root.
万方数据
维普
