Improvement of Quality Standard of Chunsheng Pills
Objective To improve the quality standard of Chunsheng Pills.Methods Lycii Fructus,Astragali Radix and Scutellariae Radix in the preparation were identified by the microscope.Cuscutae Semen,Polygoni Multiflori Radix,Epimedii Folium in the preparation were identified qualitatively by the thin-layer chromatography(TLC)method.The flavonoids in Vaccariae Semen,2,3,5,4´-tetrahydroxystilbene-2-O-β-D-glucoside,hyperoside and baicalin contents in the preparation were determined by the ultra-high-performance liquid chromatography(UPLC)method;the chromatographic column was the Acquity UPLC BEH C18 column(100 mm×2.1 mm,1.8 µm),the mobile phase was methanol-0.2%phosphoric acid aqueous solution(gradient elution),the flow rate was 0.3 mL/min,and the detection wavelengths were 280 nm(baicalin),320 nm(flavonoids in Vaccariae Semen,2,3,5,4´-tetrahydroxystilbene-2-O-β-D-glucoside),360 nm(hyperoside),and the injection volume was 2 µL.Results The microscopic characteristics of Lycii Fructus,Astragali Radix and Scutellariae Radix were obvious.The TLC chromatograms of Cuscutae Semen,Polygoni Multiflori Radix,Epimedii Folium had clear spots,good resolution,and there was no interference from the negative reference.The linear ranges of flavonoids in Vaccariae Semen,2,3,5,4´-tetrahydroxystilbene-2-O-β-D-glucoside,hyperoside and baicalin were 0.008-0.032 mg/mL,0.074-0.296 mg/mL,0.015-0.060 mg/mL,0.263-1.054 mg/mL,respectively(r>0.999 0).The RSDs of precision,stability and repeatability tests were all lower than 2.0%.The average recovery rates of flavonoids in Vaccariae Semen,2,3,5,4´-tetrahydroxystilbene-2-O-β-D-glucoside,hyperoside and baicalin were 100.87%,100.43%,98.43%,98.78%,with RSDs of 1.66%,2.44%,2.08%,1.49%respectively(n=6).Conclusion The established method is easy,which can be used for the quality control of Chunsheng Pills.
万方数据
维普
